Ontology highlight
ABSTRACT:
PROVIDER: PRJNA748567 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Purpose:Acupuncture exerts cardioprotective effect on myocardial I/R injury. In order to elucidate the potential mechanisms, RNA-seq by next generation sequencing was used to identify the rat genome-wide alterations by EA at Neiguan acupoint pretreatment in this study Methods: Adult male Sprague Dawley rats (250-300g) were divided into four groups: Sham operation(SO), I/R, electro-acupuncture at Neiguan pretreatment (EA) and electro-acupuncture at non-acupoint (NA) groups and myocardium mRNA profiles of rats in each group were generated by deep sequencing,using Illumina Hiseq 2000. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. Results: Using an optimized data analysis workflow, we mapped about 20~43 million sequence reads per sample to the mouse genome (UCSC RN4) and identified 26190 22895 25160 22852 transcripts in the SO, I/R, EA and NA rats with TopHat and Cufflinks workflow respectively. Approximately 32% and 22% of the transcripts showed differential expression between the SO and I/R, I/R and EA , I/R and NA, respectively with a |log2 fold change |≥1. Conclusions: Our results for the first time generated genome-wide gene expression profiles both in the rat I/R model and in acupuncture treatment by high throughput sequencing. The optimized data analysis workflows reported here should provide a framework for acupuncture investigations of expression profiles.
2016-03-20 | GSE61840 | GEO
Project description:Genome-wide profiling of gene expression under electro-acupuncture pretreatment on myocardial I/R injury in rats
| PRJNA262523 | ENA
Project description:To reveal the molecular signatures of XingNaoKaiQiao acupuncture on the Cerebral cortex of rats with cerebral ischemia/reperfusion injury, we performed bulk RNA sequencing.
2024-05-01 | GSE262257 | GEO
Project description:Genome-wide profiling of gene expression under electro-acupuncture treatment on MI heart in rats
| PRJNA235265 | ENA
Project description:Purpose: Acupuncture exerts cardioprotective effects on several types of cardiac injuries, especially myocardial ischemia (MI). In order to elucidate the potential mechanisms, RNA-seq by next generation sequencing was used to identify the rat genome-wide alterations after MI and EA treatment in this study Methods: Adult male Sprague Dawley rats (250-300g) were divided into three groups: Control, MI and electro-acupuncture (EA) groups, myocardium mRNA profiles of rats in each group were generated by deep sequencing,using Illumina Hiseq 2000. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: BurrowsâWheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. Results: Using an optimized data analysis workflow, we mapped about 25 million sequence reads per sample to the mouse genome (UCSC RN4) and identified 121,233, 178,242, 161,880 transcripts in the Control, MI and EA rats with TopHat and Cufflinks workflow respectively. Approximately 32% and 22% of the transcripts showed differential expression between the Control and MI, MI and EA respectively with a fold change â¥2.0. Conclusions: Our results for the first time generated genome-wide gene expression profiles both in the rat MI model and in acupuncture treatment by high throughput sequencing. The optimized data analysis workflows reported here should provide a framework for acupuncture investigations of expression profiles. Myocardium mRNA profiles of Control, MI and EA rats were generated by Hiseq 2000, Control and MI+EA group there were three samples, MI group there were two samples.
2016-03-21 | E-GEOD-54132 | biostudies-arrayexpress
Project description:Purpose: Acupuncture exerts cardioprotective effects on several types of cardiac injuries, especially myocardial ischemia (MI). In order to elucidate the potential mechanisms, RNA-seq by next generation sequencing was used to identify the rat genome-wide alterations after MI and EA treatment in this study Methods: Adult male Sprague Dawley rats (250-300g) were divided into three groups: Control, MI and electro-acupuncture (EA) groups, myocardium mRNA profiles of rats in each group were generated by deep sequencing,using Illumina Hiseq 2000. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. Results: Using an optimized data analysis workflow, we mapped about 25 million sequence reads per sample to the mouse genome (UCSC RN4) and identified 121,233, 178,242, 161,880 transcripts in the Control, MI and EA rats with TopHat and Cufflinks workflow respectively. Approximately 32% and 22% of the transcripts showed differential expression between the Control and MI, MI and EA respectively with a fold change ≥2.0. Conclusions: Our results for the first time generated genome-wide gene expression profiles both in the rat MI model and in acupuncture treatment by high throughput sequencing. The optimized data analysis workflows reported here should provide a framework for acupuncture investigations of expression profiles.
2016-03-21 | GSE54132 | GEO
Project description:We analyzed transcriptomic characteristic of motor cortex from Sprague-Dawley rats brain with cerebral ischemia and reperfusion (CIR) treated by acupuncture.
2024-01-12 | GSE222705 | GEO
Project description:Interventions: Electro-acupuncture group:Electro-acupuncture;Sham electro-acupuncture group:Sham electro-acupuncture;Routine group:Routine therapy
Primary outcome(s): The time of first postoperative anal exhaust
Study Design: Parallel
| 41280 | ecrin-mdr-crc
Project description:Exploring the effect of XingNaoKaiQiao acupuncture stimulation in rats with cerebral ischemia injury
| PRJNA1090915 | ENA
Project description:This project contains mass spectrometry-based proteomic data generated from brain tissue samples of a rat middle cerebral artery occlusion/reperfusion (MCAO/R) model. Male Sprague-Dawley rats were assigned to control, sham, model, and acupuncture groups. Quantitative proteomics based on 4D-FastDIA was performed to characterize acupuncture-associated protein abundance changes after cerebral ischemia-reperfusion injury. Bioinformatics analyses were used to explore the functional relevance of differentially abundant proteins, with a focus on mitochondrial energy metabolism, translational regulation, membrane trafficking, and synaptic plasticity. Selected candidate proteins, including Slc25a24, P4htm, Cadm2, and Arfgap1, were further validated by parallel reaction monitoring (PRM). This dataset provides a proteomic resource for investigating acupuncture-associated molecular changes in cerebral ischemia-reperfusion injury.
| PXD078874 |