Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure [Methyl-seq]

ABSTRACT: HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure [Methyl-seq]

PROVIDER: PRJNA748758 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15210200_1.fastq.gz	Fastqsanger.gz
	SRR15210200_2.fastq.gz	Fastqsanger.gz
	SRR15210201_1.fastq.gz	Fastqsanger.gz
	SRR15210201_2.fastq.gz	Fastqsanger.gz
	SRR15210202_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 30

Similar Datasets

HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure

Project description:HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure

| PRJNA749071 | ENA

HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure [ChIP-Seq]

Project description:HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure [ChIP-Seq]

| PRJNA749075 | ENA

HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure [RNA-Seq]

Project description:HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure [RNA-Seq]

| PRJNA749059 | ENA

HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure [Methyl-seq]

Project description:Recent advances in synthetic mammalian embryo models have opened new avenues to understand the complex events controlling lineage decisions and morphogenetic processes occurring during peri-implantation and early organogenesis. Two main strategies have been developed to build embryo-like structures (ELS): by assembling extraembryonic and embryonic stem cells (ESCs) or by subjecting ESCs to various morphogens. Here, we show that mouse ESCs solely exposed to chemical inhibition of SUMOylation, a posttranslational modification which acts as a general barrier to cell-fate transitions, generates early ELSs comprising both anterior neural and trunk-associated regions. HypoSUMOylated ESCs give rise to adherent spheroids which, once in suspension, self-organize into gastrulating structures containing cell types spatially and functionally related to embryonic and extraembryonic compartments. Alternatively, spheroids cultured in an optimized droplet-microfluidic device form elongated structures characterized by a multi-axial organization reminiscent of natural embryo morphogenesis. Single-cell RNA-sequencing further revealed a variety of cellular lineages including properly positioned anterior neuronal cell types, Schwann cell precursors and paraxial mesoderm segmented into somite-like structures. Mechanistically, transient SUMOylation suppression gradually increases DNA methylation genome-wide and repressive marks deposition at Nanog, enhancing ESC plasticity. Our approach provides a proof of principle for a potential new strategy to study early embryogenesis by targeting reprogramming roadblocks to shape multicellular architecture.

2023-03-17 | GSE180597 | GEO

HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure [ChIP-Seq]

2023-03-17 | GSE180680 | GEO

HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure [RNA-Seq]

2023-03-17 | GSE180676 | GEO

HypoSUMOylation in embryonic stem cells generate head-trunk embryo-like structures [scRNAseq]

Project description:HypoSUMOylation in embryonic stem cells generate head-trunk embryo-like structures [scRNAseq]

| PRJNA749070 | ENA

HypoSUMOylation in embryonic stem cells generate head-trunk embryo-like structures [ChIP-seq]

Project description:HypoSUMOylation in embryonic stem cells generate head-trunk embryo-like structures [ChIP-seq]

| PRJNA813445 | ENA

HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-03-17 | GSE180489 | GEO

Brief suppression of SUMOylation in embryonic stem cells generates embryo-like structures

Project description:Recent advances in synthetic mammalian embryo models have opened new avenues to understand the complex events controlling lineage specification and morphogenetic processes occurring during peri-implantation and early organogenesis. Two main strategies have been developed to build embryo-like structures (ELSs): by assembling extraembryonic and embryonic stem cells (ESCs) or by subjecting ESCs to various morphogens. Here, we show that mouse ESCs solely exposed to chemical inhibition of SUMOylation, a post-translational modification which acts as a general chromatin barrier to cell fate transitions, generates ELSs comprising both anterior neural and trunk-associated regions. HypoSUMOylation-instructed ESCs first give rise to adherent spheroids which, once in suspension, self-organize into gastrulating structures containing cell types spatially and functionally related to embryonic and extraembryonic compartments. Alternatively, spheroids cultured in an optimized droplet-microfluidic device form elongated structures that undergo axial organization reminiscent of natural embryo morphogenesis. Single-cell RNA-sequencing further revealed a variety of cellular lineages including properly positioned anterior neuronal cell types, Schwann cell precursors and paraxial mesoderm segmented into somite-like structures. Mechanistically, transient SUMOylation suppression gradually increases DNA methylation genome-wide and repressive marks deposition at Nanog, enhancing ESC plasticity. Our approach provides a proof of principle for a potential strategy to study early embryogenesis by targeting molecular roadblocks of cell fate change to shape multicellular architecture.

2023-04-13 | PXD031548 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data