Project description:Augmenter of liver regeneration (ALR) is a critical multi-isoform protein with its longer isoform, located in the mitochondrial intermembrane space, being part of the mitochondrial disulfide relay system (DRS). Upregulation of ALR was observed in multiple forms of cancer, among them hepatocellular carcinoma (HCC). To shed light into the ALR role in the DRS in HCC, we thus pharmacologically inhibited ALR, resulting in profound mitochondrial impairment and cancer cell proliferation deficits. These effects were mostly reversed by supplementation with bioavailable hemin b, linking the DRS, and specifically ALR function, to mitochondrial iron homeostasis. Since tumor cells are known for their increased iron demand and since increased iron levels in cancer are associated with poor clinical outcome, these results help to further advance the intricate relation between iron and mitochondria in liver cancer.
Project description:The commercially available zebrafish (Danio rerio) gene expression microarray (4x44K format, Agilent G2519F-026437) was used to characterise the changes in gene expression levels in the ovary and brain of adult female zebrafish after exposure to 55, 553 and 5442 ng/L DRS for 14 days. Also, the transcriptional response in the brain of adult female zebrafish was characterised after exposure to mixtures of DRS and P4 at concentrations of 27+0.8, 277+8, 3119+123 (ng/L), respectively. This study was designed to achieve an understanding of the mode of action of DRS and steroid mixtures in zebrafish.
Project description:To determine differential gene expression in peripheral blood of asthmatic individuals undergoing allergen inhalation challenge between early responders (ERs) and dual responders (DRs) following allergen inhalation challenge Blood was collected immediately prior to, and two hours after challenge The change in gene expression (post expression minus pre expression) in ERs was compared with the change in gene expression in DRs using age and sex as covariates Preprocessing and probeset filtering were applied to the entire dataset (28 CEL files) using the Factor Analysis for Robust MicroArray Summarization (farms) package in R The Linear Models for MicroArrays (limma) package was used to determine differential gene expression using a Benjamini Hochberg FDR of 10%.
Project description:DNA replication stress (DRS)-linked genomic instability has emerged as an important factor driving cancer development. To understand the mechanisms of DRS-associated genomic instability and phenotypic evolution, we mapped chromosomal alterations in a yeast strain with lowered expression of the replicative DNA polymerase δ. At a whole-genome level, we identified both hotspots of mitotic recombination and chromosome-specific aneuploidy dependent on decreased levels of DNA polymerase δ. The high rate of chromosome loss is likely a reflection of reduced DNA repair capacity in strains with low levels of DNA polymerase. Most recombinogenic DNA lesions were introduced during S or G2 phase, presumably as a consequence of broken replication forks.
Project description:We have identified a honeybee (Apis mellifera) odorant receptor (Or) for the queen substance 9-oxo-2-decenoic acid (9-ODA) from four candidate sex pheromone odorant receptors from the honeybee genome based on their biased expression in drone antennae. Keywords: Tissue Comparison
Project description:PC-9 cells were exposed in vitro to the reversible EGFR inhibitor ZD1839 (gefitinib) and resistant clones were isolated. One of these resistant clones (PC-9 GR4) was then exposed to the irreversible EGFR inhibitor PF00299804 and dual-resistant clones (PC-9 DRs), or those resistant to both ZD1839 and PF00299804, were isolated. In order to compare global genomic changes between PC-9, PC-9 GR, and PC-9 DR cells, we performed a genome-wide SNP analysis.
Project description:Female larvae of the honeybee (Apis mellifera) develop into either queens or workers depending on nutrition during larval development. This nutritional stimulus triggers different developmental trajectories, resulting in adults that differ in physiology, behaviour and life-span. To understand how these developmental trajectories are established we have undertaken a comprehensive analysis of differential gene expression throughout larval development. Gene expression of honeybee queen and worker larval samples was analysed at 60 hours with high-throughout sequencing
Project description:Pathogen detection microarrays analyzing honeybee samples taken after parasitization with a predatory fly, oligos correspond to specific pathogens or pathogen families of viruses, bacteria, fungi, protists, and other parasites Samples were analyzed with the E-Predict analysis package.