Project description:To investigate the altered methylation cardiac-specific NPRA-deficient mice compares to wild type, methylation level alterations were detected by Arraystar Mouse m6A Single Nucleotide resolution microarray analysis (mazF-RIP) using the myocardium from NPRA-deficient (NPRA-/-) mice and wild-type (NPRA+/+) mice as control.
Project description:To investigate the altered methylation cardiac-specific NPRA-deficient mice compares to wild type, methylation level alterations were detected by Arraystar Mouse m6A Single Nucleotide resolution microarray analysis (mazF-RIP) using the myocardium from NPRA-deficient (NPRA-/-) mice and wild-type (NPRA+/+) mice as control.
Project description:We investigated how Hippo-deficient cardiac fibroblasts (CFs) alter their cell state and interact with immune cells during cardiac fibrosis. To study the role of the Hippo pathway in CFs, we conditionally knocked out Lats1/2 specifically in the CFs of mouse hearts (Lats1/2CKO). To test the role of Hippo-deficient CF-induced macrophages, we treated the control and Lats1/2CKO mice with or without CSF1R inhibitor to block macrophage expansion and performed ST.