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ABSTRACT: Autotoxin-stress Induce Panax notoginseng to Recruit Microbiome for Alleviating Negative Plant-soil Feedback

PROVIDER: PRJNA751684 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15329000_1.fastq.gz	Fastqsanger.gz
	SRR15329000_2.fastq.gz	Fastqsanger.gz
	SRR15329001_1.fastq.gz	Fastqsanger.gz
	SRR15329001_2.fastq.gz	Fastqsanger.gz
	SRR15329002_1.fastq.gz	Fastqsanger.gz

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RNA illumina sequencing of Panax notoginseng under CK and Cd stress treatment

Project description:The total RNA were extracted from tissues of roots from several plants of Panax notoginseng under CK and Cd stress treatment by using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. The purified PCR product was sequenced using Illumina Genome Analyzer II. The qualified reads were used to study of Panax notoginseng transcriptome under CK and Cd stress treatment.

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Effect of Panax Notoginseng on gene expression in rats with cerebral ischemia-reperfusion injury

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Nitrate fertilizer alleviates ammonium toxicity by enhancing ACLA-3 in Panax notoginseng roots

Project description:Nitrogen is one of the essential elements for plant growth. NH4+ and NO3- are two major forms of absorbing element N for higher plants. In this study we found that the growth of Panax notoginseng is inhibited when only adding ammonium nitrogen fertilizer, and adding nitrate fertilizer can alleviate the toxicity caused by ammonium. We use RNA-seq to identify genes that are related to the alleviated phenotypes after introducing NO3- to Panax notoginseng roots under NH4+ stresses. Twelve RNA-seq profiles in four sample groups, i.e., control, samples treated with NH4+, samples treated with NO3- only, and treated with both NH4+ and NO3- were obtained and analyzed to identify deregulated genes in samples with different treatments. ACLA-3 gene is downregulated in NH4+ treated samples, but is upregulated in samples treated with NO3- and with both NH4+ and NO3-, which is further validated in another set of samples using qRT-PCR. Our results suggest that unbalanced metabolism of nitrogen and nitrogen is the main cause of ammonium poisoning in roots of Panax notoginseng, and NO3- may significantly upregulate the activity of ACLA-3 which subsequently enhances the citrate cycle and many other metabolic pathways in Panax notoginseng root. These potentially increase the integrity of the Panax notoginseng roots. Our results suggest that introducing NO3- fertilizer is an effective means to prevent the occurrence of toxic ammonium in Panax notoginseng root.

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Project description:High intensity of Intraspecific Interaction Exacerbates Plant-Soil Negative Feedback in Panax notoginseng -bacterium

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Transcriptomics provides novel insights into the regulatory mechanism of Panax notoginseng exosome-like nanoparticles in HSF

Project description:In current study, we observed that Panax notoginseng exosome-like nanoparticles successfully inhibited apoptosis and promoted proliferation in HSF. Moreover, transcriptomics was implemented to uncover its underlying mechanism. A total of 7924 differentially expressed genes (DEGs) were identified, with some of them enriched in apoptosis, proliferation related pathways. Intriguingly, some DEGs were annotated in skin aging development or coagulation function pathways. In view of this, we suggested Panax notoginseng exosomes may delay skin aging. Lastly, RT-qPCR confirmed the veracity and reliability of RNA-sequencing results.

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Project description:Panax notoginseng rhizosphere microbiome sequencing

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