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Systematic Identification of CRISPR-mediated Off-target Effects by CROss-seq

ABSTRACT: Systematic Identification of CRISPR-mediated Off-target Effects by CROss-seq

PROVIDER: PRJNA756912 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15564900_1.fastq.gz	Fastqsanger.gz
	SRR15564900_2.fastq.gz	Fastqsanger.gz
	SRR15564901_1.fastq.gz	Fastqsanger.gz
	SRR15564901_2.fastq.gz	Fastqsanger.gz
	SRR15564902_1.fastq.gz	Fastqsanger.gz

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Systematic Identification of CRISPR-mediated Off-target Effects by CROss-seq

Project description:We developed CROss-seq, a versatile, efficient and cost-effective method for genome-wide off-target profiling in multiple genome editing systems. Purpose: CRISPR-mediated genome editors have been widely deployed in basic research and therapeutic applications, yet the targeting specificity of these editors is still the major concern. Method to examine the genome-wide off-target effects of these editors remains lagging behind. Methods: in vivo CROss-seq, the transfected cells are labeled with N3-kethoxal for 45 minutes; in vitro CROss-seq, the genomic DNA are incubated with N3-kethoxal for 2 hours. The treated gDNA are processed with biotin click reaction, fragmentation, adapter ligation, and pull-down steps. The purified libraries were sequenced on Illumina HiSeq X Ten platform to generate paired-end reads. Results: Through CROss-seq we successfully both in vivo and in vitro to evaluate the off-target effects of CRISPR-Cas9 nuclease, base editors and prime editor. The in vivo assay was more specific while in vitro assay was more sensitive, highlighting the necessity of coupling in vivo and in vitro assays to achieve a comprehensive and precise assessment of the off-target effects. CROss-seq also revealed chromatin accessibility plays an important role in the off-target activity of the CRISPR-based editing. In addition, we identified limited genome-wide off-target sites of BE and PE in vivo, indicating that both editors were highly specific to target site.

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