Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Inappropriate use of antibiotics exacerbates inflammation through OMVs-induced pyroptosis in multidrug-resistant Klebsiella pneumoniae infection

ABSTRACT: Inappropriate use of antibiotics exacerbates inflammation through OMVs-induced pyroptosis in multidrug-resistant Klebsiella pneumoniae infection

PROVIDER: PRJNA757732 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15610194_1.fastq.gz	Fastqsanger.gz
	SRR15610194_2.fastq.gz	Fastqsanger.gz
	SRR15610195_1.fastq.gz	Fastqsanger.gz
	SRR15610195_2.fastq.gz	Fastqsanger.gz
	SRR15610196_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Inappropriate use of antibiotics exacerbates inflammation through OMVs-induced pyroptosis in multidrug-resistant Klebsiella pneumoniae infection

Project description:The inappropriate use of antibiotics is a severe public health problem worldwide, contributing to the emergence of multidrug-resistant (MDR) bacteria. To explore the possible impacts of the inappropriate use of antibiotics on the immune system, we use Klebsiella pneumoniae (K. pneumoniae) infection as an example and show that imipenem increases the mortality of mice infected by MDR K. pneumoniae. Further studies demonstrate that imipenem enhances the secretion of outer membrane vesicles (OMVs) with significantly elevated presentation of GroEL, which promotes the phagocytosis of OMVs by macrophages that depends on the interaction between GroEL and its receptor LOX-1. OMVs cause the pyroptosis of macrophages and the release of proinflammatory cytokines, which contribute to exacerbated inflammatory responses. We propose that the inappropriate use of antibiotics in the cases of infection by MDR bacteria such as K. pneumoniae might cause damaging inflammatory responses, which underlines the pernicious effects of inappropriate use of antibiotic.

2021-08-28 | GSE182766 | GEO

RNA sequencing of tumor-associated macrophages sorted from OMVs and IN@OMVs-treated mice

Project description:To investigate the effects of OMVs and IN@OMVs treatments on mouse tumor-associated macrophages, mouse tumors were harvested after treatment, and CD11b+ F4/80+ labeled cells (TAMs) were sorted by FACS for RNA sequencing.

2025-07-23 | GSE285523 | GEO

short RNA-Seq of Pseudomonas aeruginosa outer membrane vesicles (OMVs) and whole cells

Project description:Characterization of the sRNA content of P. aeruginosa OMVs compared to whole cells. Result: OMVs contain differentially packaged sRNAs. Whole cell PA14 and OMVs from 3 separate preparations.

2016-05-13 | E-GEOD-71598 | biostudies-arrayexpress

short RNA-Seq of Pseudomonas aeruginosa outer membrane vesicles (OMVs) and whole cells

Project description:Characterization of the sRNA content of P. aeruginosa OMVs compared to whole cells. Result: OMVs contain differentially packaged sRNAs.

2016-05-13 | GSE71598 | GEO

Effect of OMVs on gene expression of primary alveolar epithelial cell

Project description:The alveolar epithelial cell injury is essential for the initaitation and maintenance of inflammatory of lung in ARDS. Outer membrane vesicles (OMVs) are nanoparticles produced by the extrusion of the outer membrane of gram-negative bacteria deliver a range of bacterial molecules to host cells. Recent findings describe OMVs play vital roles in bacterial colonization, delivery of virulence factors, and disease pathogenesis. We here report OMVs as a key regulator of gene expression in primary alveolar epithelial cell.

2025-12-30 | GSE255442 | GEO

Anti-PD-1 exacerbates bleomycin-induced lung injury in mice via Caspase-3/GSDME-mediated pyroptosis

Project description:To revealed the significant involvement of GSDME-mediated pyroptosis in the pathogenesis and development of ICI-LI

2024-12-23 | GSE280068 | GEO

RNA sequencing of tumor-associated macrophages sorted from OMVs and IN@OMVs-treated mice

Project description:RNA sequencing of tumor-associated macrophages sorted from OMVs and IN@OMVs-treated mice

| PRJNA1204305 | ENA

Genetically engineered hybridized OMVs for treatment of implant-associated infections and relapse prevention through host immunomodulation

Project description:Implant-associated infections (IAIs) are refractory to elimination and local immunosuppressive microenvironment exacerbates the therapeutic difficulty, further causing the persistence and relapse. Therefore, exploring immune-strengthening therapeutics holds great promise to reverse suppressive host immunity and thoroughly eradicate chronic or repetitive infections. Bacterial outer membrane vesicles (OMVs) emerge as a potential immunostimulatory candidate, which is however, still suffer from lacking active targeting capability and non-specific systemic inflammatory storms. Herein, we genetically engineered bone marrow-derived mesenchymal stem cells (BMSCs) to express CXCR4 and isolated the cell membranes (mBMSCCXCR4) to hybridize them with OMVs derived from Escherichia coli (E. coli) to finally produce nanovesicles (mBMSCCXCR4@OMV). Due to the hybridized membrane structure of mBMSCCXCR4, the resulting nanovesicles generated excellent targeting capability towards bone marrow and were obviously uptaken by bone marrow-derived macrophages, triggering the efficient transition to pro-inflammatory M1 status through TLR/NF-κB pathway. This alteration promoted its innate bactericidal capacity and antigen presentation. Subsequent activation of T cells and B cells derived from bone marrow and inhibition of myeloid-derived suppressor cells (MDSCs) facilitated the in vivo clearance of infections through adaptive immunity via mouse models. In addition, mBMSCCXCR4@OMV also boosted memory B cells and bacteria-specific antibody responses. Together, this study warrants our mBMSCCXCR4@OMV to eradicate complicated IAIs and provides a whole-stage protection to prevent the postsurgical relapse, marking a significant immunotherapeutic advancement in the post-antibiotic era.

2025-11-30 | GSE281508 | GEO

Anti-PD-1 exacerbates bleomycin-induced lung injury in mice via Caspase-3/GSDME-mediated pyroptosis

Project description:Anti-PD-1 exacerbates bleomycin-induced lung injury in mice via Caspase-3/GSDME-mediated pyroptosis

| PRJNA1176132 | ENA

Nur77 induces GSDMC-mediated pyroptosis

Project description:Pyroptosis is a type of lytic cell death executed by members of the gasdermin family. It has been demonstrated that induction of pyroptosis can effectively inhibit tumor growth, which implicates a new strategy for clinical tumor therapy, especially for those tumors (such as melanoma) that are insensitive or resistant to the induction of apoptosis.By means of a chemical compound DBIC, designed and identified by our group recently, to induce GSDMC-mediated pyroptosis in melanoma cells.

2025-07-07 | GSE273365 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data