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Expression profiles of E4BP4 knockout RAW264.7 cells [RAW_KOvsWT_RNA-seq]

ABSTRACT: Expression profiles of E4BP4 knockout RAW264.7 cells [RAW_KOvsWT_RNA-seq]

PROVIDER: PRJNA759126 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15676420.fastq.gz	Fastqsanger.gz
	SRR15676421.fastq.gz	Fastqsanger.gz
	SRR15676422.fastq.gz	Fastqsanger.gz
	SRR15676423.fastq.gz	Fastqsanger.gz
	SRR15676424.fastq.gz	Fastqsanger.gz

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Expression profiles of E4BP4 knockout RAW264.7 cells [RAW_KOvsWT_RNA-seq]

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to evaluate the gene expression of E4BP4 knockout (KO) RAW264.7 cells. Methods: We generated E4BP4-KO RAW264.7 Cell Line using CRISPR-CAS9 system. Control plasmid was encoding the Cas9 nuclease without gRNA sequence. Total RNA of E4BP4-KO and Control (CTRL) RAW264.7 cells was extracted. RAW264.7 cells RNA profiles were generated by deep sequencing for two groups (E4BP4-KO versus CTRL RAW264.7 cells) with three samples each. Results: There were significant differences between E4BP4-KO and CTRL RAW264.7 cells. Conclusions: Deletion of E4BP4 in RAW264.7 cells induced various changes at the transcription level.

2024-03-31 | GSE183076 | GEO

Expression profiles of E4BP4 overexpression RAW264.7 cells

Project description:Expression profiles of E4BP4 overexpression RAW264.7 cells

| PRJNA978599 | ENA

Expression profiles of E4BP4 overexpression RAW264.7 cells

Project description:E4BP4 was overexpressed in RAW264.7 cells using lentivirus and RNAseq was performed.

2024-03-31 | GSE233929 | GEO

Assay for Transposase-Accessible Chromatin in E4BP4 overexpressed RAW264.7 cells

Project description:ATACseq was performed by overexpressing E4BP4 in RAW264.7 cells using lentivirus.

2024-03-31 | GSE233928 | GEO

ChIP_seq for E4BP4 in RAW264.7 cells [RAW_ChIP-seq]

Project description:ChIP_seq for E4BP4 in RAW264.7 cells [RAW_ChIP-seq]

| PRJNA759131 | ENA

Assay for Transposase-Accessible Chromatin in E4BP4 overexpressed RAW264.7 cells

Project description:Assay for Transposase-Accessible Chromatin in E4BP4 overexpressed RAW264.7 cells

| PRJNA978597 | ENA

ChIP_seq for E4BP4 in RAW264.7 cells [RAW_ChIP-seq]

2024-03-31 | GSE183079 | GEO

Hepatocyte E4BP4 promotes liver fibrosis via enhancing hepatic stellate cells activation

Project description:To investigate the role of E4bp4 during non-alcholic liver diseases, we subjected the WT mice and E4bp4 liver specific knockout (E4bp4-LKO) mice to NASH diet for 20 weeks.

2024-03-01 | GSE223518 | GEO

Expression profiles of M2-polarized RAW264.7 cells [RAW_M2vsM0_RNA-seq]

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to evaluate the gene expression of M2-polarized RAW264.7 cells compared with M0 RAW264.7 cells. Methods: RAW264.7 cells were polarized toward M2 using IL-4. M0 RAW264.7 cells were maintained in culture without IL-4. Total RNA of M2 and M0 RAW264.7 cells was extracted. RAW264.7 cells RNA profiles were generated by deep sequencing for two groups (M2 versus M0 RAW264.7 cells) with three samples each. Results: There were significant differences between M2 and M0 RAW264.7 cells. Conclusions: Polarization of RAW264.7 cells from M0 to M2 induces various changes at the transcription level.

2024-03-31 | GSE183078 | GEO

Expression profiles of M1-polarized RAW264.7 cells [RAW_M1vsM0_RNA-seq]

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to evaluate the gene expression of M1-polarized RAW264.7 cells compared with M0 RAW264.7 cells. Methods: RAW264.7 cells were polarized toward M1 using LPS and IFN-γ. M0 RAW264.7 cells were maintained in culture without LPS and IFN-γ. Total RNA of M1 and M0 RAW264.7 cells was extracted. RAW264.7 cells RNA profiles were generated by deep sequencing for two groups (M1 versus M0 RAW264.7 cells) with three samples each. Results: There were significant differences between M1 and M0 RAW264.7 cells. Conclusions: Polarization of RAW264.7 cells from M0 to M1 induces various changes at the transcription level.

2024-03-31 | GSE183077 | GEO

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