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Assay for Transposase-Accessible Chromatin in E4BP4 overexpressed RAW264.7 cells

ABSTRACT: Assay for Transposase-Accessible Chromatin in E4BP4 overexpressed RAW264.7 cells

PROVIDER: PRJNA978597 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24798242_1.fastq.gz	Fastqsanger.gz
	SRR24798242_2.fastq.gz	Fastqsanger.gz
	SRR24798243_1.fastq.gz	Fastqsanger.gz
	SRR24798243_2.fastq.gz	Fastqsanger.gz
	SRR24798244_1.fastq.gz	Fastqsanger.gz

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Assay for Transposase-Accessible Chromatin in E4BP4 overexpressed RAW264.7 cells

Project description:ATACseq was performed by overexpressing E4BP4 in RAW264.7 cells using lentivirus.

2024-03-31 | GSE233928 | GEO

Expression profiles of E4BP4 overexpression RAW264.7 cells

Project description:E4BP4 was overexpressed in RAW264.7 cells using lentivirus and RNAseq was performed.

2024-03-31 | GSE233929 | GEO

Expression profiles of E4BP4 knockout RAW264.7 cells [RAW_KOvsWT_RNA-seq]

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to evaluate the gene expression of E4BP4 knockout (KO) RAW264.7 cells. Methods: We generated E4BP4-KO RAW264.7 Cell Line using CRISPR-CAS9 system. Control plasmid was encoding the Cas9 nuclease without gRNA sequence. Total RNA of E4BP4-KO and Control (CTRL) RAW264.7 cells was extracted. RAW264.7 cells RNA profiles were generated by deep sequencing for two groups (E4BP4-KO versus CTRL RAW264.7 cells) with three samples each. Results: There were significant differences between E4BP4-KO and CTRL RAW264.7 cells. Conclusions: Deletion of E4BP4 in RAW264.7 cells induced various changes at the transcription level.

2024-03-31 | GSE183076 | GEO

Expression profiles of E4BP4 overexpression RAW264.7 cells

Project description:Expression profiles of E4BP4 overexpression RAW264.7 cells

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ChIP_seq for E4BP4 in RAW264.7 cells [RAW_ChIP-seq]

Project description:ChIP_seq for E4BP4 in RAW264.7 cells [RAW_ChIP-seq]

| PRJNA759131 | ENA

Expression profiles of E4BP4 knockout RAW264.7 cells [RAW_KOvsWT_RNA-seq]

Project description:Expression profiles of E4BP4 knockout RAW264.7 cells [RAW_KOvsWT_RNA-seq]

| PRJNA759126 | ENA

ChIP_seq for E4BP4 in RAW264.7 cells [RAW_ChIP-seq]

2024-03-31 | GSE183079 | GEO

Liver Transposase-Accessible Chromatin signal changes related to fat content in pigs

Project description:The present work emplyed pigs as an animal model for recognition of molecular processes associated with fat deposition. The Transposase-Accessible Chromatin signal changes dependent on fat content were showed at the liver levels. In the experiment Złotnicka White pigs were used that were not under selection pressure, because they are included in to National Preservation Program.

2022-04-03 | GSE199870 | GEO

Liver Transposase-Accessible Chromatin signal changes related to fat content in pigs

Project description:Liver Transposase-Accessible Chromatin signal changes related to fat content in pigs

| PRJNA821868 | ENA

ChIP-seq for E4BP4 in CD4+ T cell

Project description:Here, using ChIP-seq, we demonstrate that the transcriptional repressor Adenovirus E4 promoter-binding protein (E4BP4) binds directly to the Bcl6 promoter, which a key transcription factor controlling Tfh cell differentiation. By obtaining sequence from chromatin immunoprecipitated DNA of E4BP4 overexpressing CD4+T cells, we generated genome-wide binding gene spectrums of E4BP4. These results reveal that E4BP4 interacts with BCL6 and E4BP4 directly modulated the expression of Bcl6 to reveal the mechanism downstream of E4BP4 that regulates Tfh cell differentiation.

2021-01-19 | GSE121387 | GEO

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