Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Do nutritional profiles of floral pollen correlate with solitary bee microbiomes?

PROVIDER: PRJNA763907 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15914900_1.fastq.gz	Fastqsanger.gz
	SRR15914900_2.fastq.gz	Fastqsanger.gz
	SRR15915000_1.fastq.gz	Fastqsanger.gz
	SRR15915000_2.fastq.gz	Fastqsanger.gz
	SRR15915100_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 590

Similar Datasets

A Combined Proteomic and Metabolomic Strategy for Allergens Characterization in Natural and Fermented Brassica napus Bee Pollen

Project description:Bee pollen is consumed for its nutritional and pharmacological benefits, but it also contains hazardous allergens which has not been identified. Here, we identified 2 potential allergens, glutaredoxin and oleosin-B2, in Brassica napus (B. napus) bee pollen using mass spectrometry-based proteomics analyses, and used bioinformatics to predict their antigenic epitopes. Comparison of fermented (by Saccharomyces cerevisiae) and unfermented bee pollen samples indicated that glutaredoxin and oleosin-B2 contents were significantly decreased following fermentation, while the contents of their major constituent oligopeptides and amino acids were significantly increased based on metabolomics analyses. Immunoblot analysis indicated that the IgE-binding affinity with extracted bee pollen proteins was also significantly decreased after fermentation, suggesting a reduction in the allergenicity of fermented bee pollen. Furthermore, fermentation apparently promoted the biosynthesis of L-valine, L-isoleucine, L-tryptophan and L-phenylalanine, as well as their precursors or intermediates. Thus, fermentation could potentially alleviate allergenicity, while also positively affecting nutritional properties of B. napus bee pollen. Our findings might provide a scientific foundation for improving the safety of bee pollen products to facilitate its wider application.

2022-03-23 | MTBLS3950 | MetaboLights

Project description:Pollen microbiome and floral resourse composition of honey bee diet

| PRJNA875936 | ENA

Project description:Urban solitary bee foraging

| PRJNA790728 | ENA

Raw data from "Biotechnological approach for development and characterization of protein feed for Melipona quadrifasciata"

Project description:Analysis and comparison of the metabolomic profile of fermented pollen (colected by Melipona quadrifasciata stingless bee), fermented feed (mixture of fermented pollen with bran to feed Melipona quadrifasciata stingless bee) and bran (used in the mixture with fermented pollen). The abbreviations that name the raw files are: T7 - Fermented feed. POLEN - Fermented pollen. CTRL - Bran. FALSA - False organic solvent used (HPLC grade MeOH) and equipment system (UHPLC-HRMS).

2024-12-11 | MSV000096643 | MassIVE

Project description:Solitary bee larvae modify microbial diversity of pollen provisions in the stem-nesting bee, Osmia cornifrons (Megachilidae)

| PRJNA893014 | ENA

Nutrigenomics in honey bees: the impact of pollen nutrients on bee health

Project description:We studied the molecular mechanisms underlying the impact of pollen nutrients on honey bee (Apis mellifera) health and how those nutrients improve resistance to parasites. Using digital gene expression, we determined the changes in gene expression induced by pollen intake in worker bees parasitized or not by the mites Varroa destructor, known for suppressing immunity and decreasing lifespan of bees.

2011-11-06 | GSE25161 | GEO

Nutrigenomics in honey bees: the impact of pollen nutrients on bee health

2011-11-06 | E-GEOD-25161 | biostudies-arrayexpress

The venom profile of the solitary European carpenter bee Xylocopa violacea

Project description:We describe the first comprehensive venom profile of a solitary bee, the European carpenter bee Xylocopa violacea, by using a combined proteo-transcriptomic approach. Transcripts of the venom system are used as specific database to match peptide sequences from mass spectrometry analyses of venom from dissected venom glands and venom injected during a defensive sting. In our analyses, we only discuss the proteo-transcriptomically supported venom components and reveal a diverse venom profile with 43 different protein families of which 32 are identified in the injected venom.

2023-06-26 | PXD030997 | Pride

Developmental transcriptional profiling of Triticeae reproductive development

Project description:Despite their importance, there remains a paucity of large scale expression-based studies of reproductive development in the species belonging to the Triticeae. As a first step to address this deficiency, a gene expression atlas of triticale reproductive development was generated using the 55K Affymetrix GeneChipÂ® Wheat Genome Array. The global transcriptional profiles of the anther/pollen, ovary and stigma were analyzed at concurrent developmental stages and co-regulated as well as preferentially expressed genes were identified. Data analysis revealed both novel and conserved regulatory factors underlying Triticeae floral development and function. Triticale reproductive tissues (anther, ovary, stigma) were collected at 4 successive stages using pollen development as a developmental reference: tetrad (TET), uninucleate microspore (UNM), bi-cellular pollen (BCP), and tri-cellular pollen (TCP). Mature pollen grains (MPG) were also collected. Three biological replicates were analyzed for each tissue using the 55K Affymetrix GeneChipÂ® Wheat Genome Array.

2012-11-01 | E-GEOD-41984 | biostudies-arrayexpress

Cytological and transcriptome analysis at meiosis and mitosis reveals insights into pollen fertility in synthetic Brassica allohaploid by crosses between B. nigra and B. rapa

Project description:Purpose:The molecular mechanism of pollen abortion in interspecific hybrids of B.rapa and B.nigra was revealed by cytological and transcriptional analysis Methods:floral buds (contain two developmental progress,1.1-1.5 mm and 1.5-6.0 long floral buds) were collected, then Separated male organs were kept in liquid nitrogen immediately until use. Total RNA was extracted using the TRIzol reagent (Invitrogen, Waltham, MA, USA). DNase (Promega, USA) was used to remove potential DNA contamination. For the quantitative real-time polymerase chain reaction (qRT-PCR) analysis Results:In this study, there were extensive variations in gene expression patterns of this hybrid, and down-regulated expression of key genes for pollen development, such as meiosis of pollen mother cells, DNA damage repair and pollen sperm cell differentiation, may lead to pollen abortion. Styrene-propyl synthesis and wax biosynthesis pathway were blocked, which affected pollen wall formation. Conclusions:The wide variation of genes showed that the expression of A and B genomes were affected differently. B genome tended to downregulate the expression of key genes during meiosis and mitosis, while A genome tended to downregulate the expression of genes related to stress and pollen wall formation. The results showed that the development of male gametophyte was seriously affected after synthesis.

2023-01-06 | GSE193368 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data