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Profiling of microRNAs associated with Nucleolin (NCL) in human pulmonary artery smooth muscle cells

ABSTRACT: Profiling of microRNAs associated with Nucleolin (NCL) in human pulmonary artery smooth muscle cells

PROVIDER: PRJNA767257 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16114479.fastq.gz	Fastqsanger.gz
	SRR16114480.fastq.gz	Fastqsanger.gz
	SRR16114481.fastq.gz	Fastqsanger.gz
	SRR16114482.fastq.gz	Fastqsanger.gz

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Profiling of microRNAs associated with Nucleolin (NCL) in human pulmonary artery smooth muscle cells

Project description:Analysis of miRNAs associated with NCL via RNA immunoprecipitation in pulmonary artery smooth muscle cells using NCL antibodies to identify a distinct set of miRNAs regulated by NCL. Results provide insight into the regulatory mechanism of RNA binding proteins in vascular smooth muscle cells.

2023-03-20 | GSE184972 | GEO

microRNA profiling of human pulmonary artery smooth muscle cells under hypoxia

Project description:Analysis of hypoxia-exposed human pulmonary artery smooth muscle cells to identify the commonly regulated microRNAs by hypoxia. Results provide insight into the regulatory mechanism of hypoxic responses in vascular smooth muscle cells.

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microRNA profiling of exosomes derived from human pulmonary artery smooth muscle cells under PDGF stimulation

Project description:Analysis of exosomal microRNAs secreted by PDGF-stimulated human pulmonary artery smooth muscle cells. Results increase our understanding of exosome-mediated crosstalk between vascular cells under a pathological condition.

2020-04-22 | GSE145508 | GEO

Proteomics analysis of lncRNA Snhg18 interacting proteins in pulmonary artery smooth muscle cells

Project description:To identify proteins that interact with Snhg18, we performed the RNA pull down assay followed by mass spectrometry in pulmonary artery smooth muscle cells.

2026-05-13 | PXD070529 | Pride

Binding of Nucleolin to HBV cccDNA

Project description:Covalently closed circular DNA (cccDNA) forms the basis for replication and persistence of hepatitis B virus (HBV) in the chronically infected liver. In this study we sought to identify host factors interacting with the HBV genome. Nucleolin (Ncl) was identified as a potential interactor of HBV cccDNA. This interaction was veriefied using an established ChIPseq protocol. The data show that Ncl binds cccDNA albeit at lower levels than HBcAg. Ncl deposition occurs across the genome without a clear localization and a variable pattern of deposition between experiments. This verifies the interaction of Ncl with HBV-cccDNA.

2021-12-31 | GSE123715 | GEO

Transcriptional profiling of human pulmonary artery smooth muscle cells under hypoxia

Project description:Analysis of hypoxia-exposed human pulmonary artery smooth muscle cells to identify the commonly regulated genes by hypoxia. Results provide insight into the regulatory mechanism of hypoxic responses in vascular smooth muscle cells.

2019-09-25 | GSE118362 | GEO

Nuclear nucleolin interactome analysis

Project description:Nucleolin (NCL) is a major component of the cell nucleolus, which has the ability to rapidly shuttle to several other cell’s compartments. NCL has been shown to play important roles in a variety of essential functions among which ribosome biogenesis, gene expression and cell growth. However, the precise mechanisms underlying NCL functions are still unclear. Our study aimed to provide new information on NCL functions via the identification of its nuclear interacting partners. Using an interactomics approach, we identified 140 binding partners of NCL, among which, a 100 of them, were specifically found associated to NCL independently of the presence of RNA.

2016-04-11 | PXD003554 | Pride

Human pulmonary artery smooth muscle cells under hypoxia

Project description:Human pulmonary artery smooth muscle cells under hypoxia

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Apelin/APJ targeted microRNAs in pulmonary artery endothelial cells

Project description:Evaluation of microRNAs that are downstream of apelin/APJ signaling in the pulmonary artery endothelial cells.

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Nucleolin-aptamer therapy in retinoblastoma: molecular changes and mass spectrometry–based imaging

Project description:Retinoblastoma (RB) is an intraocular childhood tumor which, if left untreated, leads to blindness and mortality. Nucleolin (NCL) protein which is differentially expressed on the tumor cell surface, binds ligands and regulates carcinogenesis and angiogenesis. We found that NCL is over expressed in RB tumor tissues and cell lines compared to normal retina. We studied the effect of nucleolin-aptamer (NCL-APT) to reduce proliferation in RB tumor cells. Aptamer treatment on the RB cell lines (Y79 and WERI-Rb1) led to significant inhibition of cell proliferation. Locked nucleic acid (LNA) modified NCL-APT administered subcutaneously (s.c.) near tumor or intraperitoneally (i.p.) in Y79 xenografted nude mice resulted in 26 and 65% of tumor growth inhibition, respectively. Downregulation of inhibitor of apoptosis proteins, tumor miRNA-18a, altered serum cytokines, and serum miRNA-18a levels were observed upon NCL-APT treatment. Desorption electrospray ionization mass spectrometry (DESI MS)-based imaging of cell lines and tumor tissues revealed changes in phosphatidylcholines levels upon treatment. Thus, our study provides proof of concept illustrating NCL-APT-based targeted therapeutic strategy and use of DESI MS-based lipid imaging in monitoring therapeutic responses in RB.

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