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Transcriptome analysis of small intestine and liver samples of control and LCWE-induced mice

ABSTRACT: Transcriptome analysis of small intestine and liver samples of control and LCWE-induced mice

PROVIDER: PRJNA768819 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16213465_1.fastq.gz	Fastqsanger.gz
	SRR16213465_2.fastq.gz	Fastqsanger.gz
	SRR16213466_1.fastq.gz	Fastqsanger.gz
	SRR16213466_2.fastq.gz	Fastqsanger.gz
	SRR16213467_1.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of small intestine and liver samples of control and LCWE-induced mice

Project description:Purpose:Mucosal immune regulation is considered a key aspect of IgAN immunopathogenesis. Direct experimental evidence clarifying the role of intestinal mucosa attributes in IgAN is lacking. Methods:Small intestine and liver mRNA profiles in control and LCWE-induced mice were generated by deep sequencing. 2 μg total RNAs were used for stranded RNA sequencing library preparation using KC-DigitalTM Stranded mRNA Library Prep Kit for Illumina® . The library products corresponding to 200-500 bps were enriched, quantified and finally sequenced on Illumina Novaseq 6000. Results:The transcriptional signature showed that numerous genes were highly upregulated in the small intestines of the LCWE-induced mice compared with those in PBS mice. GO analysis of the small intestine revealed an over-representation of genes involved in the Fc gamma R-mediated phagocytosis, B cell receptor signaling pathway, NF-κB signaling pathway, and intestinal immune network for IgA production. The GO analysis of live samples revealed that genes involved in metabolic pathways, galactose metabolism, drug metabolism-other enzymes, and bile secretion were downregulated Conclusions:The intestinal barrier was dysfunctional in the LCWE-induced mice and liver’s ability to clear IgA may be reduced.

2022-02-11 | GSE185367 | GEO

Human small intestine