Project description:Over 8000 high-qualified CD45IV-DAPI-CD45+ dura cells were sorted for single cell RNA sequencing; 13 cell populations were identified, Including T cells, B cells ,NK cells, monocytes, erythroid cells, and progenitors.
Project description:Dural metastasis (DM) is a rarely researched disease with limited therapeutic options, largely due to our circumscribed understanding of dura mater. However, recently emerging studies suggest the dura mater is an immune hub and implements immunosurveillance of the central nervous system. These evidences imply potential immunotherapies of DM. Here, we established a preclinical murine model of DM. We have employed a single cell sequencing approach using 10x Genomics scRNA-seq to study the tumor microenvironment of DM model. In comparison, the dura maters of nontumor mice and leptomeningeal metastasis (LM) mice were used as control groups. Briefly, the dorsal dura maters are isolated from the head of nontumor mice, 4T1-LM mice and 4T1-DM mice on day 12 since tumor cell inoculation, and are enzymatically digested to single cells with collagenase VIII and DNase I.
Project description:To further elucidate molecular differences in immune cells and identify potential sources of Vegfa in response to photothrombotic injury, we performed single-cell RNA-sequencing of CD45+ cells isolated from the mouse dura mater or brain after photothrombotic injury.
Project description:We performed single cell RNA sequencing on peripheral blood leukocytes from BALB/c mice and IL1rn-deficient mice treated with vehicle or rapamycin
Project description:We used single-cell RNA sequencing to characterize the heterogeneity of circulating leukocytes in dogs, then employed the dataset to investigate how primary osteosarcoma (OS) tumors impacted circulating leukocytes.