Project description:We use ChIP-seq targeting histone 3 lysine 4 mono-methylation (H3K4me1) to identify putative enhancer sites genome-wide, in the retrosplenial cortex of adult prairie vole males. ChIP samples were generated by targeting a known enhancer mark (H3K4me1) in chromatin extracted from the retrosplenial cortex of 8 males. Illumina libraries were prepared from ChIP and INPUT DNA and sequenced on Illimuna HiSeq 2500 platform.
Project description:Orthohantaviruses are rodent-borne viruses that can cause severe disease in humans but remain asymptomatic in their natural hosts. Increasing outbreaks and ecological changes raise important public health concerns. Understanding how these viruses interact with host cells is essential, particularly in reservoir species. Using RNA sequencing, the study compares cellular responses to different orthohantaviruses in bank vole kidney and respiratory cells. The results identify shared and virus- or tissue-specific regulatory factors linked to infection outcomes. Note that biological material originates from immortalized epithelial cell lines derived from bank vole (Myodes glareolus, NCBI Taxonomy ID: 447135). Mus musculus was selected in controlled vocabulary fields due to repository limitations and because mouse orthologs were used for functional annotation.
Project description:More than 40 species of mammal have been reported to be infected naturally with Schistosoma japonicum (Chinese mainland strain) in China. The reed vole, Microtus fortis, is the only known mammalian host in which the schistosomes are unable to mature and cause significant pathogenic changes. Gene expression profiling of the 10 day old schistosomula was performed. Microarray analysis was also used to identify differences in gene expression between Schistosoma japonicum schistosomula from BALB/c mice and from Microtus fortis. 10 day old schistosomula were isolated, total RNA obtained and Agilent one colour labeling used. A custom designed Agilent microarray was used to determine what differential gene expression occurs between parasites maintained in either a permissive (mouse) or non-permissive (vole) hosts.
Project description:The expression levels of developing vole molar and jaw transcriptome were measured. each of first molars at embryonic days 13-16 (E13, E14, E15, E16), second molars at E16, and jaw tissues at E14.
Project description:More than 40 species of mammal have been reported to be infected naturally with Schistosoma japonicum (Chinese mainland strain) in China. The reed vole, Microtus fortis, is the only known mammalian host in which the schistosomes are unable to mature and cause significant pathogenic changes. Gene expression profiling of the 10 day old schistosomula was performed.
