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Human OA cartilages treated with ANGPTL3 mimetic LNA043 and placebo

ABSTRACT: Human OA cartilages treated with ANGPTL3 mimetic LNA043 and placebo

PROVIDER: PRJNA773037 | ENA |

REPOSITORIES: ENA

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Human OA cartilages treated with ANGPTL3 mimetic LNA043 and placebo

Project description:Human OA patients subject to total knee replacement (TKR) were randomly selected and treated either LNA043 or placebo via intra-articular injection at 2hrs, 7days and 21days before surgery. The cartilage tissue were collected. Total RNA were extraced from both damaged and undamaged looking part of the tissue, and underwent gene expression profiling performed using next-generation sequencing on a Illumina platform.

2022-09-08 | GSE186220 | GEO

Microarray identifies differentially expressed mRNAs of cartilages between osteoarthritis (OA) patients and control patients

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2026-03-22 | GSE308380 | GEO

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ANGPTL3 in podocytes contributes to pathogenesis of lupus nephritis by activating MSR1 in macrophage

Project description:Lupus nephritis (LN), a severe complication of systemic lupus erythematosus (SLE), is driven by immune complex deposition and inflammatory renal injury. This study uncovers a novel mechanism in which podocyte-derived angiopoietin-like protein 3 (ANGPTL3) exacerbates LN pathogenesis through macrophage scavenger receptor 1 (MSR1)-mediated activation. Protein interaction screening revealed direct binding between ANGPTL3 and MSR1, a macrophage-enriched receptor. Clinical analyses demonstrated elevated glomerular expression of ANGPTL3 and MSR1 in LN patients, correlating with reduced renal function and histopathological damage. In vitro, ANGPTL3-overexpressing podocytes activated macrophages in an MSR1-dependent manner. RNA sequencing of ANGPTL3-stimulated bone marrow-derived macrophages (BMDMs) identified robust activation of interferon signaling pathways, with key genes (Tnip3, Isg20). Silencing Msr1 abolished these effects, suppressing interferon-related gene expression. In the pristane-induced lupus model, ANGPTL3 and MSR1 co-localized with infiltrating macrophages in injured glomeruli, mirroring human LN pathology. These findings establish the ANGPTL3-MSRI axis as a critical driver of macrophage activation and interferon signaling in LN, highlighting ANGPTL3 as a promising therapeutic target to mitigate immune-mediated renal injury in SLE.

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The effect of ANGPTL3 mAb on liver transcriptomes in LDLR-deficient mice

Project description:Purpose: The goal of this study was to evaluate the effect of ANGPTL3 inhibition on liver transcriptomes in LDLR KO mice. Methods: Mice were treated once weekly for 3 weeks with 25mg/kg control mAb or ANGPTL3 mAb. Livers were harvested 6 days after the last mAb treatment. Results: ANGPTL3 inhibition had no major impact on hepatic gene expression.

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ANGPTL3/8 is an atypical unfoldase that regulates intravascular lipolysis by catalyzing unfolding of lipoprotein lipase as measured by hydrogen/deuterium exchange mass spectrometry (HDX-MS)

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2025-03-26 | PXD060125 | Pride

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