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N1-methylpseudouridine found within COVID-19 mRNA vaccines produces faithful protein products

ABSTRACT: N1-methylpseudouridine found within COVID-19 mRNA vaccines produces faithful protein products

PROVIDER: PRJNA773947 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16549500_1.fastq.gz	Fastqsanger.gz
	SRR16549500_2.fastq.gz	Fastqsanger.gz
	SRR16549501_1.fastq.gz	Fastqsanger.gz
	SRR16549501_2.fastq.gz	Fastqsanger.gz
	SRR16549502_1.fastq.gz	Fastqsanger.gz

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N1-methylpseudouridine found within COVID-19 mRNA vaccines produces faithful protein products

Project description:The COVID mRNA vaccines utilize the modified nucleobase N1-methylpseudouridine, in place of canonical uridine, to improve immunogenicity and protein yield. However, relatively few studies have investigated the effect of modified nucleobases on the fidelity of protein translation. Given the interest in the COVID mRNA vaccines, we sought to investigate how N1-methylpseudouridine (and the related modification pseudouridine) is read by ribosomes.

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Project description:Given the interest in the COVID mRNA vaccines, we sought to investigate how the RNA modification N1-methylpseudouridine (and its related modification, pseudouridine) is read by ribosomes and reverse transcriptases. By looking at reverse transcriptase data, we can gain information on how the modification affects duplex stability, which may have important consequences for the tRNA-mRNA interactions found in the ribosome.

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N1-methylpseudouridine found within COVID-19 mRNA vaccines produces faithful protein products.

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