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ABSTRACT: Carbapenemase-producing Enterobacteriaceae Genome sequencing and assembly

PROVIDER: PRJNA774102 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
		Other
	SRR16572838_1.fastq.gz	Fastqsanger.gz
	SRR16572838_2.fastq.gz	Fastqsanger.gz
	SRR16572839_1.fastq.gz	Fastqsanger.gz
	SRR16572839_2.fastq.gz	Fastqsanger.gz

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Project description:Antibiotic resistance associated with the expression of the clinically significant carbapenemases, IMP, KPC, and NDM and OXA-48 in Enterobacteriaceae is emerging as a worldwide calamity to health care. In Australia, IMP-producing Enterobacteriaceae is the most prevalent carbapenemase-producing Enterobacteriaceae (CPE). Genomic characteristics of such carbapenemase-producing Enterobacteriaceae (CPE) are well described, but the corresponding proteome is poorly characterised. We have thus developed a method to analyse dynamic changes in the proteome of CPE under antibiotic pressure. Specifically, we have investigated the effect of meropenem at sub-lethal concentrations to develop a better understanding of how antibiotic pressure leads to resistance. Escherichia coli, producing either NDM, IMP or KPC type carbapenemase were included in this study, and their proteomes were analysed in growth conditions with or without meropenem.

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