Project description:This project contains single-cell RNA sequencing (scRNA-seq) data of the ovarian stroma from the viviparous teleost, black rockfish (Sebastes schlegelii). To define how the ovarian stroma is reprogrammed across reproduction, we profiled stromal cells spanning three distinct biological states: unfertilized, normal-gestation, and abnormal-gestation (characterized by spontaneous embryonic mortality and avascularity). This dataset aims to characterize the stromal cell composition, developmental trajectories, and immune-vascular interactions critical for maintaining intraovarian gestation, providing insights into the evolutionary biology of teleost viviparity.
2026-04-19 | GSE324772 | GEO
Project description:Transcriptome data of juvenile black rockfish liver tissue after exposure to fiber-type MP
| PRJNA1053507 | ENA
Project description:Hepatic transcriptomic analysis in black rockfish
Project description:Comparisons of gene expression profiles between testis and ovary of juvenile and wild brooders were made through a cDNA microarray in the black tiger shrimp (Penaeus monodon). Differentially expressed genes were identified through the microarray analysis, and the microarray results were confirmed by real-time PCR. Selected genes were further characterized.
Project description:We sequenced complementary DNA created from white muscle messenger RNA in juvenile blue rockfish and performed a differential gene expression analysis to describe the fishes' responses to the global change-related stressors of high pCO2 (low pH) and hypoxia (low dissolved oxygen, DO). To examine gene expression over ecologically realistic timescales that emulate the duration of spring upwelling events in the California Current ecosystem, we collected and sequenced samples after 12 h, 24 h and two weeks of exposure to four treatments: control (pH≈8.0, pCO2≈400μatm, DO≈8 mg/L), high pCO2 (pH≈7.6, pCO2≈1200μatm, DO≈8 mg/L), hypoxic (pH≈8.0, pCO2≈400μatm, DO≈4 mg/L), and combined high pCO2/hypoxic (pH≈7.6, pCO2≈1200μatm, DO≈4 mg/L).