Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression of DREAM complex-related inducible knockouts in HeLa cells

ABSTRACT: Gene expression of DREAM complex-related inducible knockouts in HeLa cells

PROVIDER: PRJNA779723 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR16920702_1.fastq.gz	Fastqsanger.gz
	SRR16920702_2.fastq.gz	Fastqsanger.gz
	SRR16920703_1.fastq.gz	Fastqsanger.gz
	SRR16920703_2.fastq.gz	Fastqsanger.gz
	SRR16920704_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 26

Similar Datasets

Gene expression of DREAM complex-related inducible knockouts in HeLa cells

Project description:The DREAM complex and interacting proteins (e.g., BMYB and FOXM1) together control cell cycle-dependent gene expression. We identified an unexpected, specific kinetochore defect phenotype after depletion of a DREAM complex member (LIN52) in optical pooled CRISPR knockout screens of essential genes. Here we generated individual doxycycline-inducible Cas9 cell lines expressing lentiviral-integrated sgRNAs targeting LIN52, MYBL2, and FOXM1 to evaluate differential roles in gene expression regulaion.

2021-11-22 | GSE188631 | GEO

Gene expression of ZNF335, RNPC3, CLP1 and LIN52 inducible knockouts in HeLa cells

Project description:Gene expression of ZNF335, RNPC3, CLP1 and LIN52 inducible knockouts in HeLa cells

| PRJNA821912 | ENA

H3K4me3 profiling of Kdm1a/Kdm5c double-inducible forebrain-specific knockouts

Project description:H3K4me3 profiling of Kdm1a/Kdm5c double-inducible forebrain-specific knockouts

| PRJNA1255570 | ENA

The DREAM complex functions as conserved master regulator of somatic DNA repair capacities

Project description:Mutations in the DREAM complex induce germline-like gene expression patterns including the increase of multiple mechanisms of DNA repair in the soma of Caenorhabditis elegans. This confers mutants of the DREAM complex resistance to a wide range of DNA damage types during development and aging.

2023-01-31 | PXD033836 | Pride

H3K4me3 profiling of Kdm1a/Kdm5c double-inducible forebrain-specific knockouts

Project description:Loss or reduced expression of lysine demethylases (KDMs) is linked to neurodevelopmental disorders and intellectual disability. Given the phenotypic similarities between KDM1A and KDM5C deficient mice, and the convergence of both enzymes in maintaining a repressive state via H3K4 demethylation, we examined their functional interaction using double-inducible, forebrain-specific knockouts (dKDM-ifKOs). These mice showed transcriptional and epigenetic dysregulation beyond the additive effects of individual knockouts, including stronger ectopic expression of non-neuronal genes in hippocampal neurons. Thousands of de novo H3K4me3-enriched regions emerged, indicating synergistic disruption of chromatin regulation. In line with these molecular changes, dKDM-ifKOs displayed more severe behavioral impairments than the single ifKOs, along with altered hippocampal expression of ion channels and increased excitability of CA1 pyramidal neurons. These findings underscore the joint role of ID-linked KDMs in regulating cell-type-specific gene silencing and H3K4 methylation levels to safeguard neuronal identity and responsiveness, as well as cognitive function

2025-08-22 | GSE295672 | GEO

The DREAM complex antagonizes WDR5a and represses the productive elongation of transcription in Arabidopsis [GRO-seq]

Project description:The master transcriptional repressor DREAM (dimerization partner, RB-like, E2F and multi-vulval class B) complex regulates cell cycle in eukaryotes, but much remains unknown about how it transmits repressive signals on chromatin to the primary transcriptional machinery (e.g., Pol II). Through a genetic screen, we identified BTE1 (barrier of transcription elongation 1), a key component in the plant DREAM complex. The subsequent characterization demonstrated that the DREAM complex represses Pol II elongation. The core DREAM subunits E2F transcription factors recruit BTE1 to accumulate at the promoter-proximal regions of target genes. DREAM target genes exhibit characteristic enrichment of H2A.Z and H3K4me2 modification on chromatin. The DREAM complex BTE1 subunit interacts with WDR5a, repressing WDR5a chromatin binding and the productive elongation of transcription on BTE1-targeted genomic loci. Thus, we demonstrate a major role of DREAM complex in the control of transition from transcription initiation to productive elongation around TSSs on the open chromatin via inhibiting WDR5a function.

2022-06-28 | GSE181488 | GEO

The DREAM complex antagonizes WDR5a and represses the productive elongation of transcription in Arabidopsis [ChIP-seq]

2022-06-28 | GSE181486 | GEO

The DREAM complex antagonizes WDR5a and represses the productive elongation of transcription in Arabidopsis [RNA-seq]

2022-06-28 | GSE181484 | GEO

Transcriptomics analysis of the hippocampus in Kdm1a/Kdm5c double-inducible forebrain-specific knockouts

2025-08-22 | GSE295536 | GEO

Transcriptomics analysis of the hippocampus in Kdm1a/Kdm5c double-inducible forebrain-specific knockouts

Project description:Transcriptomics analysis of the hippocampus in Kdm1a/Kdm5c double-inducible forebrain-specific knockouts

| PRJNA1255146 | ENA