Ontology highlight
ABSTRACT:
PROVIDER: PRJNA780771 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR16954700.fastq.gz | Fastqsanger.gz | |||
| SRR16954701.fastq.gz | Fastqsanger.gz | |||
| SRR16954702_subreads.fastq.gz | Fastqsanger.gz | |||
| SRR16954703_subreads.fastq.gz | Fastqsanger.gz | |||
| SRR16954704_subreads.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 42 |
Similar Datasets
Project description:Streptomyces coelicolor normally produce spores with a relatively high heterogeneity, which will produce genetically heterogeneous sub-populations. These sub-populations often exert massive chromosome amplifications and deletions. Cells with gross chromosomal changes produce an increased diversity of secondary metabolites and secrete significantly more antibiotics; however, these changes come at the cost of dramatically reduced individual fitness, providing direct evidence for a trade-off between secondary metabolite production and fitness. We propose that antibiotic production in colonies of the multicellular bacterium Streptomyces coelicolor is coordinated by a division of labour. This proteomics survey will provide more detailed insights into how these chromosomal changed strains behave under normal growth condition.
2020-05-26 | PXD014413 | Pride
Project description:We identified genome-wide binding regions of NdgR in Streptomyces coelicolor using chromatin immunoprecipitation sequencing (ChIP-seq). We constructed 6×myc-tagged NdgR strain using homologous recombination with myc-tagging vector. Analysis of the sequencing data aligned to Streptomyces coelicolor genome database (NC_003888).
2014-12-29 | GSE59010 | GEO
Project description:To identify unique gene expression in cAMP supplemented Streptomyces coelicolor M1146 strain. The genes with different gene expression might be key genes to understand the effects of cAMP supplementation on the transcriptome of Streptomyces coelicolor M1146.
2020-11-27 | GSE158811 | GEO
Project description:To identify unique gene expression in cAMP supplemented Streptomyces coelicolor M145 strain. The genes with different gene expression might be key genes to understand the effects of cAMP supplementation on the transcriptome of Streptomyces coelicolor M145.
2020-11-27 | GSE158810 | GEO
Project description:We report the DNA binding profile of a novel family of nucleoid-associated protein in Streptomyces coelicolor
2022-04-02 | GSE165795 | GEO
Project description:This study compared the genome of Streptomyces rimosus rimosus against that of Streptomyces coelicolor. It also compared 4 strains with changes in oxytetracycline production and derived from G7, the type strain, against G7. Keywords: Comparative genomic hybridization
2008-01-11 | GSE10126 | GEO
Project description:A complex programme of regulation governs gene expression during development of the morphologically and biochemically complex eubacterial genus Streptomyces. Earlier work has suggested a model in which 'higher level' pleiotropic regulators activate 'pathway-specific' regulators located within chromosomal gene clusters encoding biosynthesis of individual antibiotics. We used mutational analysis and adventitious overexpression of key Streptomyces coelicolor regulators to investigate functional interactions among them. We report here that cluster-situated regulators (CSRs) thought to be pathway-specific can also control other antibiotic biosynthetic gene clusters, and thus have pleiotropic actions. Surprisingly, we also find that CSRs exhibit growth-phase-dependent control over afsR2/afsS, a 'higher level' pleiotropic regulatory locus not located within any of the chromosomal gene clusters it targets, and further demonstrate that cross-regulation by CSRs is modulated globally and differentially during the S. coelicolor growth cycle by the RNaseIII homologue AbsB. Our results, which reveal a network of functional interactions among regulators that govern production of antibiotics and other secondary metabolites in S. coelicolor, suggest that revision of the currently prevalent view of higher-level versus pathway-specific regulation of secondary metabolism in Streptomyces species is warranted. Groups of assays that are related as part of a time series. Keywords: time_series_design
2006-02-02 | GSE4138 | GEO
Project description:This work was carried out to elucidate the proteins that are regulated by the two-component system CutRS in Streptomyces coelicolor M145 and how this response changes in the presence of glucose. A comparison of the whole cell proteomes of Streptomyces coelicolor M145 WT and Streptomyces coelicolor M145 ∆cutRS on both DNA (no glucose) and DNAD (with glucose) was made.
2023-06-16 | PXD040579 | Pride
Project description:A complex programme of regulation governs gene expression during development of the morphologically and biochemically complex eubacterial genus Streptomyces. Earlier work has suggested a model in which 'higher level' pleiotropic regulators activate 'pathway-specific' regulators located within chromosomal gene clusters encoding biosynthesis of individual antibiotics. We used mutational analysis and adventitious overexpression of key Streptomyces coelicolor regulators to investigate functional interactions among them. We report here that cluster-situated regulators (CSRs) thought to be pathway-specific can also control other antibiotic biosynthetic gene clusters, and thus have pleiotropic actions. Surprisingly, we also find that CSRs exhibit growth-phase-dependent control over afsR2/afsS, a 'higher level' pleiotropic regulatory locus not located within any of the chromosomal gene clusters it targets, and further demonstrate that cross-regulation by CSRs is modulated globally and differentially during the S. coelicolor growth cycle by the RNaseIII homologue AbsB. Our results, which reveal a network of functional interactions among regulators that govern production of antibiotics and other secondary metabolites in S. coelicolor, suggest that revision of the currently prevalent view of higher-level versus pathway-specific regulation of secondary metabolism in Streptomyces species is warranted.
2005-12-02 | E-SMDB-3554 | biostudies-arrayexpress
Project description:Borodina2005 - Genome-scale metabolic network
of Streptomyces coelicolor (iIB711)
This model is described in the article:
Genome-scale analysis of
Streptomyces coelicolor A3(2) metabolism.
Borodina I, Krabben P, Nielsen
J.
Genome Res. 2005 Jun; 15(6):
820-829
Abstract:
Streptomyces are filamentous soil bacteria that produce more
than half of the known microbial antibiotics. We present the
first genome-scale metabolic model of a representative of this
group--Streptomyces coelicolor A3(2). The metabolism
reconstruction was based on annotated genes, physiological and
biochemical information. The stoichiometric model includes 819
biochemical conversions and 152 transport reactions, accounting
for a total of 971 reactions. Of the reactions in the network,
700 are unique, while the rest are iso-reactions. The network
comprises 500 metabolites. A total of 711 open reading frames
(ORFs) were included in the model, which corresponds to 13% of
the ORFs with assigned function in the S. coelicolor A3(2)
genome. In a comparative analysis with the Streptomyces
avermitilis genome, we showed that the metabolic genes are
highly conserved between these species and therefore the model
is suitable for use with other Streptomycetes. Flux balance
analysis was applied for studies of the reconstructed metabolic
network and to assess its metabolic capabilities for growth and
polyketides production. The model predictions of wild-type and
mutants' growth on different carbon and nitrogen sources agreed
with the experimental data in most cases. We estimated the
impact of each reaction knockout on the growth of the in silico
strain on 62 carbon sources and two nitrogen sources, thereby
identifying the "core" of the essential reactions. We also
illustrated how reconstruction of a metabolic network at the
genome level can be used to fill gaps in genome annotation.
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2015-07-30 | MODEL1507180049 | BioModels