Dataset Information

Proteomics survey of Streptomyces coelicolor strains with natural gross chromosome change

ABSTRACT: Streptomyces coelicolor normally produce spores with a relatively high heterogeneity, which will produce genetically heterogeneous sub-populations. These sub-populations often exert massive chromosome amplifications and deletions. Cells with gross chromosomal changes produce an increased diversity of secondary metabolites and secrete significantly more antibiotics; however, these changes come at the cost of dramatically reduced individual fitness, providing direct evidence for a trade-off between secondary metabolite production and fitness. We propose that antibiotic production in colonies of the multicellular bacterium Streptomyces coelicolor is coordinated by a division of labour. This proteomics survey will provide more detailed insights into how these chromosomal changed strains behave under normal growth condition.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Streptomyces Coelicolor Bacteria

SUBMITTER: Chao DU

LAB HEAD: Gilles P. van Wezel

PROVIDER: PXD014413 | Pride | 2020-05-26

REPOSITORIES: Pride

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Publications

Antibiotic production in Streptomyces is organized by a division of labor through terminal genomic differentiation.

Zhang Zheren Z Du Chao C de Barsy Frédérique F Liem Michael M Liakopoulos Apostolos A van Wezel Gilles P GP Choi Young H YH Claessen Dennis D Rozen Daniel E DE

Science advances 20200115 3

One of the hallmark behaviors of social groups is division of labor, where different group members become specialized to carry out complementary tasks. By dividing labor, cooperative groups increase efficiency, thereby raising group fitness even if these behaviors reduce individual fitness. We find that antibiotic production in colonies of Streptomyces coelicolor is coordinated by a division of labor. We show that S. coelicolor colonies are genetically heterogeneous because of ampl ...[more]

PMID: 31998842

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Project description:Borodina2005 - Genome-scale metabolic network of Streptomyces coelicolor (iIB711) This model is described in the article: Genome-scale analysis of Streptomyces coelicolor A3(2) metabolism. Borodina I, Krabben P, Nielsen J. Genome Res. 2005 Jun; 15(6): 820-829 Abstract: Streptomyces are filamentous soil bacteria that produce more than half of the known microbial antibiotics. We present the first genome-scale metabolic model of a representative of this group--Streptomyces coelicolor A3(2). The metabolism reconstruction was based on annotated genes, physiological and biochemical information. The stoichiometric model includes 819 biochemical conversions and 152 transport reactions, accounting for a total of 971 reactions. Of the reactions in the network, 700 are unique, while the rest are iso-reactions. The network comprises 500 metabolites. A total of 711 open reading frames (ORFs) were included in the model, which corresponds to 13% of the ORFs with assigned function in the S. coelicolor A3(2) genome. In a comparative analysis with the Streptomyces avermitilis genome, we showed that the metabolic genes are highly conserved between these species and therefore the model is suitable for use with other Streptomycetes. Flux balance analysis was applied for studies of the reconstructed metabolic network and to assess its metabolic capabilities for growth and polyketides production. The model predictions of wild-type and mutants' growth on different carbon and nitrogen sources agreed with the experimental data in most cases. We estimated the impact of each reaction knockout on the growth of the in silico strain on 62 carbon sources and two nitrogen sources, thereby identifying the "core" of the essential reactions. We also illustrated how reconstruction of a metabolic network at the genome level can be used to fill gaps in genome annotation. This model is hosted on BioModels Database and identified by: MODEL1507180049. To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

Dataset Information

Proteomics survey of Streptomyces coelicolor strains with natural gross chromosome change

Publications

Antibiotic production in <i>Streptomyces</i> is organized by a division of labor through terminal genomic differentiation.

Similar Datasets

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