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Alteration of gene expression by diallyl trisulfide (DATS) administration in chemically-induced rat mammary tumors

ABSTRACT: Alteration of gene expression by diallyl trisulfide (DATS) administration in chemically-induced rat mammary tumors

PROVIDER: PRJNA783932 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17048790_1.fastq.gz	Fastqsanger.gz
	SRR17048790_2.fastq.gz	Fastqsanger.gz
	SRR17048791_1.fastq.gz	Fastqsanger.gz
	SRR17048791_2.fastq.gz	Fastqsanger.gz
	SRR17048792_1.fastq.gz	Fastqsanger.gz

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Alteration of gene expression by diallyl trisulfide (DATS) administration in chemically-induced rat mammary tumors

Project description:We report altered gene expression profiles by DATS in chemically-induced rat mammary tumors

2021-12-03 | GSE189640 | GEO

Gene Expression Changes by Diallyl Trisulfide Administration in Chemically-induced Mammary Tumors in Rats.

Project description:Diallyl trisulfide (DATS) was shown to be a potent inhibitor of luminal-type MCF-7 xenograft growth in vivo. The present study was conducted to determine the preventive effect of DATS administration using an N-methyl-N-nitrosourea (MNU)-induced rat mammary tumor model, which shares molecular resemblance to luminal-type human breast cancers. The DATS administration (50 mg/kg body weight, 5 times/week) was safe, but did not reduce mammary tumor latency, incidence, burden or multiplicity. Therefore, we conducted RNA-seq analysis using mammary tumors from control and DATS-treated rats (n = 3 for each group) to gain insights into lack of mammary tumor prevention by this phytochemical. The gene ontology and the Kyoto encyclopedia of genes and genomes pathway analyses of the RNA-seq data revealed upregulation of genes associated with ribosomes, translation, peptide biosynthetic/metabolic process, and oxidative phosphorylation but downregulation of genes associated with mitogen-activated protein kinases. A total of 33 genes associated with ribosomes were significantly upregulated by DATS treatment, including RPL11 and RPS14. Western blotting confirmed upregulation of RPL11 and neurofascin protein expression in mammary tumors from DATS-treated rats when compared to controls. A statistically significant increase in protein level of c-Jun N-terminal kinase 2 was also observed in tumors from DATS-treated rats when compared to controls. On the other hand, expression of complex I subunits NDUFV1 or NDUFS1 was not affected by DATS treatment. These results offer potential explanations for ineffectiveness of DATS in the chemically-induced rat mammary tumor model. Inhibitors of the proteins upregulated by DATS may be needed to improve chemopreventive efficacy of this phytochemical.

| S-EPMC8984650 | biostudies-literature

Toxicological safety evaluation of diallyl trisulfide through acute and subacute toxicity studies in ICR mice

Project description:Diallyl trisulfide (DATS) is one of the main sulfide-containing compounds in garlic oil and has been shown to have a variety of biological activities such as anti-tumor, inhibition of inflammation, and immune regulation. However, there is very little information available regarding its toxicological safety. To elucidate the toxicity mechanism and find the early warning biomarker of DATS, this study conducted acute and subacute toxicity experiments according to the OECD guidelines. The potential serum biomarker and toxicity mechanism of DATS were explored by proteomics.

2024-03-28 | PXD051045 |

Mechanistic Targets of Diallyl Trisulfide (DATS) in Human Breast Cancer Cells Identified by RNA-seq Analysis

Project description:We report differential gene expression changes by DATS in breast cancer cells

2021-04-30 | GSE173616 | GEO

Cancer-Selective Mechanistic Targets of Diallyl Trisulfide (DATS) in Human Breast Cancer Cells Identified by RNA-seq Analysis

Project description:We report differentially expressed genes by DATS exposure in MCF-10A human epithelial cell line and SK-BR-3 human breast cancer cell line

2022-04-20 | GSE200872 | GEO

Mechanistic Targets of Diallyl Trisulfide (DATS) in Human Breast Cancer Cells Identified by RNA-seq Analysis

Project description:Mechanistic Targets of Diallyl Trisulfide (DATS) in Human Breast Cancer Cells Identified by RNA-seq Analysis

| PRJNA726289 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of control of Pseudomonas aeruginosa PAO1 and DATS-treated P. aeruginosa PAO1 Transcriptomes

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare transcriptome profiling of control of P. aeruginosa PAO1 (RNA-seq) to transcriptome profiling of DATS-treated P. aeruginosa PAO1 and to evaluate protocols for optimal high-throughput data analysis. Methods:LB medium (50 mL) was inoculated with exponential growth phase P. aeruginosa PAO1 at a concentration of 1.0E8 CFU/mL. Diallyl trisulfide was then added at a concentration of either 0 (control) or 0.63 μl/mL, respectively, in triplicate. All nine experiment groups were incubated in a water bath shaker at 37 ºC with a shaking rate of 180 rpm for 5 h. Cells were then sampled and centrifuged from the three control groups and three farnesol treatment groups, respectively. The cell precipitates were separately snap-frozen at -80ºC. Total RNA was isolated from cells using Trizol (Life Technologies, USA) according to the manufacturer’s protocol. Results: The transcriptome sequencing data of control and DATS-treated groups were compared and analyzed in the form of the following: control vs DATS. The details of the differentially expressed genes among the two groups showed that the amount of differential expressed genes between control group and DATS-treated group was high (total 5572, up-regulated 929, down-regulated 1432). Conclusions:

2021-12-31 | GSE178622 | GEO

Cancer-Selective Mechanistic Targets of Diallyl Trisulfide (DATS) in Human Breast Cancer Cells Identified by RNA-seq Analysis

Project description:Cancer-Selective Mechanistic Targets of Diallyl Trisulfide (DATS) in Human Breast Cancer Cells Identified by RNA-seq Analysis

| PRJNA827109 | ENA

The sulfur stress response caused by sodium hydrosulfide, allicin and diallyl polysulfanes in Bacillus subtilis as revealed by transcriptomics

Project description:In this study, we compared the transcriptome signatures in response to sodium hydrosulfide (NaHS), allicin and diallyl tetrasulfane (DAS4) exposure in the Gram-positive bacterium Bacillus subtilis. This analysis revealed that NaHS, allicin and DAS4 caused a similar thiol-specific oxidative and electrophile stress response as well as protein and DNA damage.

2019-11-18 | GSE132981 | GEO

Antimicrobial effect and the mechanism of diallyl trisulfide against Campylobacter jejuni

Project description:RNA seq analysis after treated with DATS in Campylobacter

| PRJEB42739 | ENA