Project description:A marine bacterial isolate, Brevibacterium casei was grown in LB broth in presence of 50ppm Cadmium (provided in the form cadmium chloride, with final concentration of Cadmium at 50ppm). The cells were harvested at different time intervals and proteins extracted from these using indigenous protocol developed in laboratory. Similarly, the strain was grown in absence of Cadmium and same steps were followed, which was treated as control. The proteins extracted from test as well control set were digested using trypsin following standard protocol. Trypsin digests were injected in LCMS QToF for obtaining spectral library which was used to identify the proteins against NCBI database

2026-07-31 | PXD010546 | Pride

Effect of Calcium Chloride Concentrations on the Transcriptome of Brevibacterium lines

Project description:A urease positive marine actinobacterium Brevibacterium lines was demonstrated to form and dissolve calcite precipitation in conditions with different concentration of Ca2+. Next-generation sequencing (NGS) was used to analyze the transcriptome of B. lines under 0, 50 and 150 mM Ca2+ after 24 h incubation to discover the differentially expressed genes involved. Results provide insight into the molecular response of B. lines stressed with different concentration of Ca2+.

2020-01-16 | GSE93668 | GEO

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