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CUT&Tag-seq for H3K27ac in hematopoietic stem cells in bone marrow after 5-FU administration

ABSTRACT: CUT&Tag-seq for H3K27ac in hematopoietic stem cells in bone marrow after 5-FU administration

PROVIDER: PRJNA785987 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17123920.fastq.gz	Fastqsanger.gz
	SRR17123921.fastq.gz	Fastqsanger.gz
	SRR17123922.fastq.gz	Fastqsanger.gz
	SRR17123923.fastq.gz	Fastqsanger.gz
	SRR17123924.fastq.gz	Fastqsanger.gz

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CUT&Tag-seq for H3K27ac in hematopoietic stem cells in bone marrow after 5-FU administration

Project description:Purpose: The goals of this study are to elucidate the underlying mechanism for the regulation of HSC divisions. Methods: Before or after wild type (WT) mice were treated with 5-fluorouracil (5-FU), 12500 cells of HSCs were sorted. These cells were subjected to CUT&Tag-seq to analyze H3K27ac profile by using Next-seq. The sequence reads that passed quality filters were analyzed by Trimmomatic.

2022-01-19 | GSE190148 | GEO

Hematopoietic stem cells in bone marrow after 5-FU administration

Project description:Hematopoietic stem cells in bone marrow after 5-FU administration

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ATAC-seq of hematopoietic stem cells in bone marrow after 5-FU administration

Project description:ATAC-seq of hematopoietic stem cells in bone marrow after 5-FU administration

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ATAC-seq of hematopoietic stem cells in bone marrow after 5-FU administration

Project description:Purpose: The goals of this study are to elucidate the underlying mechanism for the regulation of HSC divisions. Methods: Before or after wild type (WT) mice were treated with 5-fluorouracil (5-FU), 15000 cells of HSCs were sorted. These cells were subjected to ATAC-seq using Next-seq. The sequence reads that passed quality filters were analyzed by FASTX-toolkit.

2022-01-19 | GSE164281 | GEO

H3K27ac CUT&Tag iEC reprogramming

Project description:H3K27ac CUT&Tag iEC reprogramming

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mRNA profiles of hematopoietic stem or progenitor cells in bone marrow after 5-FU administration

Project description:mRNA profiles of hematopoietic stem or progenitor cells in bone marrow after 5-FU administration

| PRJNA689800 | ENA

mRNA profiles of hematopoietic stem or progenitor cells in bone marrow after 5-FU administration

Project description:Purpose: The goals of this study are to elucidate the underlying mechanism for the regulation of HSC divisions. Methods: Before or after wild type (WT) mice were treated with 5-fluorouracil (5-FU), 100 cells of HSCs were sorted. Moreover, after HSCs were cultured for 1day, 100 cells of phenotypic HSCs were sorted. These cells were subjected to mRNA sequence using Next-seq. The sequence reads that passed quality filters were analyzed by CLC genomic workbench.

2022-01-19 | GSE164283 | GEO

CUT&Tag of H3K27Ac of HCT26-1T: Control and GSK126

Project description:CUT&Tag for H3K27Ac; Colorectal cancer patient derived organoids, HCT26-1T; drug experiment of control vs GSK126.

2025-11-20 | E-MTAB-16203 | biostudies-arrayexpress

H3K27ac CUT&Tag iEC reprogramming

Project description:H3K27ac CUT&Tag analysis of Sox17-Erg and Etv2 reprogramming of cardiac fibroblasts into endothelial cells

2024-03-22 | GSE218415 | GEO

H3K9ac and H3K27ac Cut&Tag profiling of wild type and Rbm25-deficient Bone Marrow Derived macrophage

Project description:Purpose: To transcriptome widely reveal histone acetylation modification H3K9ac and H3K27ac regulation by Rbm25, we performed Cleavage Under Targets and Tagmentation (CUT&Tag) analysis of wild-type (WT) and Rbm25-deficiency BMDMs. Methods: Mouse BMDMs were generated from bone marrow cells in RPMI-1640 medium with recombinant mouse M-CSF (20ng/ml). BMDMs were stained to confirm the surface expression of CD11b and F4/80. Cells with purity >97.5% were used for subsequent experiments. WT and Rbm25 deficient BMDMs were stimulated with LPS (100ng/ml) for 4 hours. Results: We carried out CUT&Tag assays using antibody against H3K9ac or H3K27ac, and found the global influence of Rbm25 in regulating H3K9ac and H3K27ac of transcription regulatory regions of key genes for inflammatory macrophage effector function.

2024-11-06 | GSE240158 | GEO

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