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Cotton (Gossypium hirsutum L.) root transcriptional response to the southern Root-Knot Nematode (RKN) Meloidogyne incognita infestation

ABSTRACT: Cotton (Gossypium hirsutum L.) root transcriptional response to the southern Root-Knot Nematode (RKN) Meloidogyne incognita infestation

PROVIDER: PRJNA787300 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17173033_1.fastq.gz	Fastqsanger.gz
	SRR17173033_2.fastq.gz	Fastqsanger.gz
	SRR17173034_1.fastq.gz	Fastqsanger.gz
	SRR17173034_2.fastq.gz	Fastqsanger.gz
	SRR17173035_1.fastq.gz	Fastqsanger.gz

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Cotton (Gossypium hirsutum L.) root transcriptional response to the southern Root-Knot Nematode (RKN) Meloidogyne incognita infestation

Project description:As an initial step to explore the cotton (Gossypium hirsutum L.) root transcriptional response to the southern Root-Knot Nematode (RKN) Meloidogyne incognita infestation, conventional heirloom G. hirsutum (Gh) cultivars [susceptible Acala SJ-2 (SJ2), moderately resistant Upland Wild Mexico Jack Jones (WMJJ), and resistant Acala NemX] that have been shown to be useful as an informative genetic model for detecting and introgressing RKN resistance genes into commercial Upland cotton were used to enlighten the molecular mechanisms and gene expression of RKN resistance. Using the next generation sequencing (NGS) Illumina MiSeq and HiSeq, we performed RNA-seq profiling in roots with disease progression of 10 days and collected from 23 days old plants of SJ2, WMJJ, and NemX. With three biological replicates of each treatment from each cultivar, plants were subjected to RKN-infestation and non-infested control developing a total of 18 RNA-seq libraries

2022-04-01 | GSE190503 | GEO

Below ground attack by root knot nematode Meloidogyne graminicola predisposes rice to blast disease

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Biological Control of Root-Knot Nematode Meloidogyne incognita Infection of Tomato (Solanum lycopersicum L.) by the Oomycete Biocontrol Agent Pythium oligandrum

Project description:The biocontrol agent Pythium oligandrum, which is a member of phylum Oomycota, can control diseases caused by a taxonomically wide range of plant pathogens, including fungi, bacteria, and oomycetes. However, whether P. oligandrum could control diseases caused by plant root-knot nematodes (RKNs) was unknown. We investigated a recently isolated P. oligandrum strain GAQ1, and the P. oligandrum CBS530.74 strain, for the control of RKN Meloidogyne incognita infection of tomato (Solanum lycopersicum L.). Initially, P. oligandrum culture filtrates were found to be lethal to M. incognita second-stage juveniles (J2s) with up to 84% mortality at 24 h after treatment compared to 14% in the control group. Consistent with the lethality to M. incognita J2s, tomato roots treated with P. oligandrum culture filtrates reduced the attraction of nematodes, and the number of nematodes penetrating the roots was reduced by up to 78%. In a greenhouse pot trial, P. oligandrum GAQ1 inoculation of tomato plants significantly reduced the gall number by 58% in plants infected with M. incognita. Notably, P. oligandrum GAQ1 mycelial treatment significantly increased tomato plant height (by 36%), weight (by 27%), and root weight (by 48%). Transcriptome analysis of tomato seedling roots inoculated with the P. oligandrum GAQ1 strain identified ~2,500 differentially expressed genes. The enriched GO terms and annotations in the up-regulated genes suggested modulation of plant hormone-signaling and defense-related pathways in response to P. oligandrum. In conclusion, our results support that P. oligandrum GAQ1 can serve as a potential biocontrol agent for M. incognita control in tomato. Multiple mechanisms appear to contribute to the biocontrol effect involving direct inhibition of M. incognita, potential priming of tomato plant defenses, and plant growth promotion.

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Project description:Root-knot nematodes (RKNs), Meloidogyne spp., severely challenge plants worldwide and especially perennials. In Prunus spp., the genetic resistance to RKNs mainly relies on NLR (NB-LRR) genes that are key factors for pathogens control. In Prunus cerasifera, the Ma gene, cloned in 2011, is characterized by a complete-spectrum and heat-stable resistance that has not been overcome under field and controlled conditions up-to-now. This resistance occurs relatively early in the interaction process as RKN penetrate the roots but never succeed in inducing their feeding site and die in the root. Besides its rare ability, the Ma gene has a peculiar TNL structure comprised of five repeated post-LRR domains (a.k.a. C-JID), which is unique among plants. Conversely to other TNL in model plants, no transcriptional analyses related to this gene have been performed so far. In this study, we explore the early transcriptional response that occurs in both, susceptible (no Ma gene) and resistant (Ma-containing) Prunus cerasifera accessions facing a strong inoculum pressure of Meloidogyne incognita, the model species for RKN. Our RNAseq approach allows us to better understand the transcriptional response of a woody perennial in both the compatible and incompatible interactions with RKN, thus paving the way for future functional studies.

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Expression data from LCM-captured Arabidopsis root cells after challenge with Meloidogyne incognita

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Expression data from LCM-captured Zea mays root cells after challenge with Meloidogyne incognita

Project description:The differentiation of specialized feeding sites in Zea mays root cells in response to nematode infestation involves substantial cellular reprogramming of host cells that is not well characterized at the molecular level. Expression data was generated from Zea mays root cells undergoing giant cell formation due to nematode infestation and from non-infested control root cells. Cells were laser captured 14 and 21 days after infestation.

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Expression data from LCM-captured Arabidopsis root cells after challenge with Meloidogyne incognita

Project description:The differentiation of specialized feeding sites in Arabidopsis root cells in response to nematode infestation involves substantial cellular reprogramming of host cells that is not well characterized at the molecular level. Expression data was generated from Arabidopsis root cells undergoing giant cell formation due to nematode infestation and from non-infested control root cells. Cells were laser captured 14 and 21 days after infestation. Samples, collected 14 days post infestation, consisted of three biological replicates per treatment (control root cells or giant cells). RNA samples were isolated from ~150 control cells or from ~80 giant cells using the PicoPure RNA Isolation Kit (Arcturus, Mountain View, USA). RNA amplifications were carried out with the NuGEN WT-Ovation Pico kit. GSM546568-GSM546577: Control and giant cells collected 21 days post infestation.

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