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TRained Immunity induced by Bcg in UroThElial carcinoma (Tribute)

ABSTRACT: TRained Immunity induced by Bcg in UroThElial carcinoma (Tribute)

PROVIDER: PRJNA787479 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17179923_1.fastq.gz	Fastqsanger.gz
	SRR17179923_2.fastq.gz	Fastqsanger.gz
	SRR17179924_1.fastq.gz	Fastqsanger.gz
	SRR17179924_2.fastq.gz	Fastqsanger.gz
	SRR17179925_1.fastq.gz	Fastqsanger.gz

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TRained Immunity induced by Bcg in UroThElial carcinoma (Tribute) associated epigenetic remodeling

Project description:Trained immunity is the phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to certain microbial components, altering their responses to future exposures. Intravesical instillation with Bacillus Calmette-Guérin (BCG) is the most effective adjuvant therapy in patients with high-risk non-muscle invasive bladder cancer (HR-NMIBC). HR-NMIBC is associated with a high risk of tumor recurrence and progression to muscle-invasive bladder cancer. The precise immunological mechanisms through which BCG mediates anti-tumor immunity are still unclear. The aim of our study was to investigate the (long-term) induction of trained immunity by repeated BCG instillations in NMIBC patients and elucidate the immunological and epigenetic mechanisms that are involved. Another aim was to assess the relationship between trained immunity response and clinical response of NMIBC patients, in terms of recurrence free survival and progression free survival. To determine whether BCG instillations induce trained immunity in peripheral blood mononuclear cells (PBMCs) we performed a prospective cohort study (‘Tribute’) and isolated PBMCs collected before BCG therapy and at 8 time points during BCG therapy. A total of 17 BCG-naïve HR-NMIBC patients were included. After isolation of PBMCs, monocytes were further purified using an isolation procotol with a percoll gradient. RNA was isolated from these purified monocytes were and used as input for RNA-seq analysis.

2023-03-30 | GSE190528 | GEO

TRained Immunity induced by Bcg in UroThElial carcinoma (Tribute) associated epigenetic remodeling

Project description:TRained Immunity induced by Bcg in UroThElial carcinoma (Tribute) associated epigenetic remodeling

| PRJNA787484 | ENA

TRained Immunity induced by Bcg in UroThElial carcinoma (Tribute) associated RNA-seq analysis

2023-03-30 | GSE190529 | GEO

TRained Immunity induced by Bcg in UroThElial carcinoma (Tribute) associated RNA-seq analysis

Project description:TRained Immunity induced by Bcg in UroThElial carcinoma (Tribute) associated RNA-seq analysis

| PRJNA787483 | ENA

Stronger induction of trained immunity by mucosal BCG or MTBVAC vaccination

Project description:Vaccination against tuberculosis by intradermal Bacillus Calmette-Guérin (BCG) injection saves many lives, supposedly by inducing adaptive immune memory in lymphocytes. Epidemiologically, BCG vaccination is also associated with reduced childhood mortality unrelated to TB, which is attributed to innate immune memory, also termed trained immunity. We recently demonstrated improved protection against tuberculosis infection in highly susceptible rhesus macaques by mucosal BCG vaccination, correlating with a unique local but no peripheral immune profile. Here, we investigated local and peripheral innate immune function after intradermal versus mucosal vaccination with M. bovis BCG or the live attenuated, M. tuberculosis-derived candidate, MTBVAC. The results demonstrate an augmented frequency of trained immunity in monocytes after respiratory mucosal administration of live attenuated mycobacterial vaccines compared to intradermal immunization, with MTBVAC being equally potent as BCG. These results provide further support to strategies for improving TB vaccination and, more broadly, modulating innate immunity via mucosal surfaces.

2021-01-27 | GSE159046 | GEO

Transcriptomes during monocyte-to-macrophage differentiation and BCG induced trained immunity in vitro

2025-04-30 | GSE262098 | GEO

BCG-trained macrophages couple LDLR upregulation to type I IFN responses and anti-viral immunity

Project description:Trained immunity refers to memory-like responses of innate immune cells when they re-encounter pathogenic stimuli. Bacillus Calmette-Guérin (BCG) vaccination implies enhanced antiviral immunity whereas the underlying mechanisms remain unclear. Herein, we have uncovered elevated expression of low-density lipoprotein receptor (LDLR) on BCG-trained macrophages with robust type I interferon (IFNI) production and antiviral effects both in vivo and in vitro. Consequently, cholesterol is accumulated in BCG-trained macrophages, leading to the augmentation of NFE2L1 expression and the formation of NFE2L1/IRAK1/TRIM25 complex where TRIM25 mediates IRAK1 K63 polyubiquitination to exaggerate IFNI responses in a RIG-I dependent manner.

2025-02-27 | PXD061241 |

Effect of Lactate dehydrogenase inhibitor (LDHi; GSK2837808A) on BCG vaccine induced trained immunity in vitro

2025-04-30 | GSE262099 | GEO

Time-resolved transcriptomes of BCG vaccine induced trained immunity in human monocytes

2022-08-02 | GSE168468 | GEO

Single-cell transcriptomic profiles reveal changes associated with BCG-induced trained immunity and protective effects in circulating monocytes

Project description:Bacillus Calmette-Guérin (BCG) vaccine is one of the most widely-used vaccines worldwide. In addition to protection against tuberculosis, BCG confers a degree of non-specific protection against other infections by enhancing secondary immune responses to heterologous pathogens, an effect termed trained immunity. To better understand BCG-induced immune reprogramming, we performed single-cell transcriptomic measurements before and after BCG vaccination using secondary immune stimulation with bacterial lipopolysaccharide (LPS). We find that BCG vaccination reduces systemic inflammation, and we identify 75 genes with an altered response to LPS, including several inflammatory mediators such as CCL3 and CCL4 which have a heightened response. Co-expression analysis reveals gene modules containing these cytokines lose coordination after BCG vaccination. Others have increased coordination, including several humanin nuclear isoforms which we confirmed induce trained immunity in vitro. Our results link in vivo BCG administration to single cell transcriptomic changes, validated in human genetics experiments, and highlight new genes which may be responsible for the non-specific protective effects of BCG.

2021-11-16 | GSE184241 | GEO

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