Project description:Analysis of gene expression level. The hypothesis tested in the present study was that erf4 mutant affects PMEI genes expression which inhibited PME activity in the seed coat mucilage
Project description:Chia seeds were hydrated for 1 hr to extract mucilage and then freeze dried. Mucilage was separated and proteins extracted, digested and processed for nanoLC-MS analysis.
Project description:Transcriptome analysis on aerial roots at two different growth stages before and after mucilage production confirmed the expression of genes involved in polysaccharide synthesis and degradation. Genes related to nitrogen uptake and assimilation were up-regulated upon water exposure.
Project description:Detection of the Marmara Sea Mucilage Microbiome by Shotgun Metagenome Sequencing and Investigation of Potential Microorganisms as Key Elements in the Microbiome Network
Project description:The Zygnematophyceae are the closest algal relatives of land plants and hence interesting to understand land plant evolution. Species of the genus Serritaenia have an aerophytic lifestyle and form colorful, mucilaginous capsules, which surround the cells and block harmful solar radiation. Under laboratory conditions the production of this “sunscreen mucilage” can be induced by ultraviolet B radiation. The present dataset reveals insights into the cellular reaction of this alga to UV radiation (a major stressor in terrestrial habitats) and allows for comparisons with other algae and land plants to draw evolutionary conclusions.