Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A single cell analysis of porcine thymopoiesis and thymic iNKT cells

ABSTRACT: A single cell analysis of porcine thymopoiesis and thymic iNKT cells

PROVIDER: PRJNA791835 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR17317700.fastq.gz	Fastqsanger.gz
	SRR17317701.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

A single cell analysis of porcine thymopoiesis and thymic iNKT cells

Project description:Many aspects of the porcine immune system remain poorly characterized which poses a barrier to improving swine health and utilizing pigs as preclinical models. Here, we employed single-cell RNA sequencing (scRNA-seq) to create a cell atlas of the postnatal pig thymus. Our data found conserved features as well as species-specific differences in cell states and cell types compared to human thymocytes. We also describe several unconventional T cell types with gene expression profiles associated with innate effector functions. This includes a cell census of >11,000 differentiating invariant natural killer T (iNKT) cells, which revealed that the functional diversity of pig iNKT cells differs substantially from the paradigm of iNKT0/1/2/17 subset differentiation established in mice. Collectively, our data characterizes key differentiation events in porcine thymopoiesis and iNKT cell maturation, which provide new insights into pig T cell development.

2022-08-04 | GSE192520 | GEO

Single-cell RNA transcriptomics of thymic iNKT cells from C57BL/6 and Hivep3-/- mice

Project description:Single-cell RNA-seq analysis of murine thymic iNKT cells from two independent C57BL/6 mice and two independent Hivep3-deficient mice

2020-11-06 | GSE152786 | GEO

scTCR-seq of thymic iNKT cells

Project description:iNKT cells show significant lineage diversity among mouse strains. We used scTCR-seq to analyze the diversity of thymic iNKT cells between BALB/c and B6 mice.

2026-01-21 | GSE298294 | GEO

scRNA-seq of thymic iNKT cells

Project description:iNKT cells show significant lineage diversity among mouse strains. We used scRNA-seq to analyze the diversity of thymic iNKT cells between BALB/c and B6 mice.

2026-01-21 | GSE298293 | GEO

Single-cell RNA transcriptomics of thymic iNKT cells from BALB/c and Cd80/Cd86 -/- (B7KO) mice

Project description:Single-cell RNA-seq analysis of murine thymic iNKT cells from three independent BALB/c mice and three independent Cd80/Cd86 (B7)-deficient mice

2022-11-29 | GSE198967 | GEO

Single-cell TCR transcriptomics of thymic iNKT cells from BALB/c and Cd80/Cd86 -/- (B7KO) mice

Project description:Single-cell TCR-seq analysis of murine thymic iNKT cells from three independent BALB/c mice and three independent Cd80/Cd86 (B7)-deficient mice

2022-11-29 | GSE198966 | GEO

Recapitulation of murine thymopoiesis in vitro from single hematopoietic stem cells

Project description:We report a serum-free, 3-D murine artificial thymic organoid (ATO) system that mimics normal murine thymopoiesis from four different mouse strains with the production of all T cell populations, from early thymic progenitors to functional single positive (CD8SP and CD4SP) TCRaband TCRyd cells. RNA sequencing aligned ATO-derived populations with phenotypically identical primary thymocytes. ATOs initiated with Rag1-/- marrow produced the same differentiation block as seen in the endogenous thymus, and Notch signaling patterns in ATOs mirrored primary thymopoiesis. ATOs initiated with defined hematopoietic stem cells (HSCs) and progenitors from marrow and thymus recapitulated subsequent stages and the normal kinetics of T cell differentiation. Remarkably, a single HSC deposited into ATOs generated a complete trajectory of T cell differentiation producing a similar diverse TCR repertoire across clones. ATOs represent a technically simple, highly reproducible and powerful experimental platform for the study of clonal thymopoiesis from HSCs.

2020-12-15 | GSE146224 | GEO

Thymic iNKT cells in Prkd2/3 mice (scRNA/TCR-seq, scATAC-seq)

Project description:We have conducted single cell-RNA, -TCR, -ATAC sequencing (10x Genomics) to investigate a difference of gene expression, TCR repertoir and chromatin accesibility of thymic iNKT cells from wild-type and CD4-Cre-mediated T cell-specific Prkd2/3 doubly deficirnt (Prkd2/3ΔCD4) mice. Results demonstrate that Prkd2/3 are required for iNKT cell development and formaion of proper iNKT cell subtypes.

2025-09-08 | GSE288015 | GEO

The Effect of PRMT5 deficiency on thymic iNKT cells

Project description:Protein arginine methylation is a post-translational modification catalyzed by protein arginine methyltransferase (PRMT). To elucidate the role of PRMT5 in T cells, we generated T-cell specific PRMT5-deficient mice (Prmt5 flox/d Cd4-Cre mice) and found a severe loss of thymic iNKT cells as well as a reduced number in peripheral CD4+ and CD8+ T cells. As iNKT cells were significantly decreased in the stage 1, 2 and 3 of developmental stages, RNA-seq was performed using stage 1 iNKT cells of control and PRMT5-deficient mice. This transcriptome analysis will provide mechanistic insight into how PRMT5 contributes to thymic iNKT cell development.

2018-08-06 | GSE118135 | GEO

scRNA-seq of thymic iNKT cells

Project description:scRNA-seq of thymic iNKT cells

| PRJNA1268898 | ENA