Project description:We established two iPSC-derived 3D models recapitulating human somitogenesis, Somitoids and Segmentoids. Somitoids recapitulate the temporal sequence of somitogenesis, with all cells undergoing differentiation and morphogenesis in a synchronous manner. On the other hand, Segmentoids recapitulate the spatio-temporal features of somitogenesis, including gene expression dynamics, tissue elongation, sequential somite morphogenesis, and polarity patterning. They therefore provide an excellent proxy to study human somitogenesis.
Project description:This SuperSeries is composed of the following subset Series:; GSE7012: Identif. of oscillatory genes in somitogenesis from functional genomic analysis of C2C12 myoblast line; GSE7015: Identif. of oscillatory genes in somitogenesis from functional genomic analysis of a human mesenchymal stem cell model Experiment Overall Design: Refer to individual Series
Project description:In this project, we characterized the temporal dynamics of protein expression during the differentiation of human iPSCs to somitoids, stem cell–derived 3D models that recapitulate early embryonic somitogenesis. Using quantitative time-course proteomics, we captured how protein abundance changed as pluripotent stem cells progressed into segmented, organized structures, providing insight into the molecular processes underlying human axial development.
Project description:This project performs quantitative proteomics analysis on 9 human pluripotent stem cells (hPSCs) by using the 4D-FastDIA technology. There are 3 groups, and each group has 3 biological replicates. The goal is to map the proteome of hPSCs, which helps to deeply investigate their molecular mechanisms at the protein level.