Project description:MiRNA arrays were performed for the plasma exosomes of ESCC patients before treatment. The tumor regression grade of the primary tumor (TRG-PT) was used to evaluate the therapeutic effect of chemoradiotherapy (CRT). Patients classified as Grade 3 were considered sensitive to CRT (n=4), while those classified as Grade 0 or 1a were considered resistant to CRT (n=4).
Project description:To identify the candidate exosomal miRNAs involved in ESCC metastasis, we conducted small RNA sequencing to determine the miRNA expression profiles of plasma exosomes from 5 LNM+ and 5 LNM- ESCC patients
Project description:To investigate the differences in microRNA expression in plasma exosomes among normal individuals, patients with type 2 diabetes, we employed Agilent microarray expression profiling as a discovery platform to detect changes in microRNA levels in different target populations.
Project description:microRNA profiles of Exosomes from Pooled NPC Patients serum comparing Control Exosomes from Healthy donors serum Two-condition experiment, Exosomes from Pooled Healthy donors serum vs. Exosomes from Pooled NPC Patients serum. Biological replicates: 1 Exosomes from Pooled Healthy donors serum, 1 Exosomes from Pooled NPC Patients serum,
Project description:Exosomes play a crucial role in the occurrence and development of tumors. To investigate the levels of different exosome expressions in the plasma of liver cancer patients before and after liver tumor resection, we conducted high-throughput sequencing of miRNA in preoperative and postoperative blood exosomes from liver cancer patients.
Project description:Despite a significant progress in the treatment of Acute Respiratory Distress Syndrome (ARDS), our ability to identify early patients and predict outcome remains limited. In this study, we aimed to characterize small RNA content of plasma exosomes from ARDS patients in order to identify potential diagnostic biomarkers of the disease. For the first time, we profiled miRNA expression levels in plasma-derived exosomes from ARDS patients (n=8) compared to healthy subjects (n=10) by small RNA-seq. It allowed us to identify 12 exosomal miRNAs differentially expressed in ARDS context (padj<0.05).
Project description:Exosomes are extracellular vesicles that have been shown to have therapeutic effects. However, the role of plasma-derived exosomal miRNA in premature ovarian failure (POF) remains ambiguous. To investigate the epigenetic pathogenesis in POF, we analyzed the expression profile of miRNA in exosomes derived from plasma of health control and POF patients using RNA sequencing.
Project description:In order to detect the expression profile of plasma microRNAs, we have employed microRNA microarray expression profiling as a discovery platform to identify microRNAs with the potential to distinguish the different microRNA profiles from ESCC patients and healthy controls. Three pairs of plasma samples from ESCC patients and healthy controls without any digestive tract disease history were collected for microarray analysis.