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The VarA-CsrA regulatory pathway influences cell shape in Vibrio cholerae

ABSTRACT: The VarA-CsrA regulatory pathway influences cell shape in Vibrio cholerae

PROVIDER: PRJNA803738 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17893371.fastq.gz	Fastqsanger.gz
	SRR17893372.fastq.gz	Fastqsanger.gz
	SRR17893373.fastq.gz	Fastqsanger.gz
	SRR17893374.fastq.gz	Fastqsanger.gz
	SRR17893375.fastq.gz	Fastqsanger.gz

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The VarA-CsrA regulatory pathway influences cell shape in Vibrio cholerae

Project description:In this study, we determined the expression profile of V. cholerae strain A1552 and its varA-deficient mutant at two different growth stages (OD600 of 0.2 or 2.5)

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The VarA-CsrA regulatory pathway influences cell shape in Vibrio cholerae.

Project description:Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood. This pathway, which mainly signals through the downstream protein CsrA, is highly conserved among gamma-proteobacteria, indicating there is likely a broader function of this system beyond virulence regulation. In this study, we investigated the VarA-CsrA signaling pathway and discovered a previously unrecognized link to the shape of the bacterium. We observed that varA-deficient V. cholerae cells showed an abnormal spherical morphology during late-stage growth. Through peptidoglycan (PG) composition analyses, we discovered that these mutant bacteria contained an increased content of disaccharide dipeptides and reduced peptide crosslinks, consistent with the atypical cellular shape. The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired. Importantly, this phenotype, and the overall cell rounding, could be prevented by means of cell wall recycling. Collectively, our data provide new insights into how V. cholerae use the VarA-CsrA signaling system to adjust its morphology upon unidentified external cues in its environment.

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