Project description:To determine the cellular effect of iron oxide nanoparticles on MSC, we performed gene expression microarray assay to explore more intensive molecular basis.
Project description:Dissimilatory iron reduction by hyperthermophilic archaea occurs in many geothermal environments and generally relies on microbe-mineral interactions that transform various iron oxide minerals. In this study, the physiology of dissimilatory iron and nitrate reduction was examined in the hyperthermophilic crenarchaeon Pyrodictium delaneyi Su06T. Protein electrophoresis showed that the c-type cytochrome and general protein compositions of P. delaneyi changed when grown on ferrihydrite relative to nitrate. Differential proteomic analyses using tandem mass tagged protein fragments and mass spectrometry detected 660 proteins and differential production of 127 proteins. Among these, two putative membrane-bound molybdopterin-dependent oxidoreductase complexes increased in relative abundance 60- to 3,000-fold and 50-100-fold in cells grown on iron oxide. A putative 8-heme c-type cytochrome was 60-fold more abundant in iron grown cells and was unique to the Pyrodictiaceae. There was also a >14,700-fold increase in a membrane transport protein in iron grown cells. There were no changes in the abundances of flagellin proteins nor a putative nitrate reductase, but a membrane nitric oxide reductase was more abundant on nitrate. These data help to elucidate the mechanisms by which hyperthermophilic crenarchaea generate energy and transfer electrons across the membrane to iron oxide minerals.
Project description:The purpose of this study was to identify genes that are differentially expressed upon M. tuberculosis exposure to nitric oxide (NO) or iron limitation stresses, either individually or in combination.
Project description:The expression ovarian cancer cell line HeyA8 was studied when the cells were treated with MFH at 43˚C for 30 min, the iron oxide nanoparticles concentration was 0.5 mgFe/ml.
Project description:Magnetically-actuated iron oxide nanoparticles (MNP) have emerged as a technology to chronically expose axons of developing neurons to extremely low mechanical forces. This force was found to induce axon outgrowth. Axon-Seq has been performed to profile local gene expression in response to the stimulus.
2022-03-07 | GSE197808 | GEO
Project description:PFASs removal in iron oxide and FeRB based CWs
Project description:To identify differentially expressed genes and key biological pathways that define toxicity following nanomaterial exposure, we performed microarray analyses on NR8383 macrophages exposed for 4 h to 17 µg/mL of zinc iron oxide (ZnFe2O4, NRCWE021). This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement N°. 686098
Project description:We compared the gene expression analysis of 2 different glomerular isolation techniques (laser capture microdissection with 2 rounds of RNA amplification and unamplified glomerular RNA after iron perfusion with glomerular sieving) and obtained different results depending on the glomerular isolation technique that was used Experiment Overall Design: Paired kidneys from Lewis-Hsd rat kidneys were collected. The glomeruli of one kidney was obtained by LCM with 2 rounds of RNA amplification while the unamplified glomerular RNA of the contralateral kidney was obtained after perfusion with iron oxide and isolated by magnetic purification and a glomerular sieving technique. The RNA was analyzed using the Affymetrix Rat 230A microarrays.