Project description:transcriptome sequencing was performed to analyze the gene expression profiles of Eriocheir sinensis under normal temperature (22°C) and high temperature (27°C, 32°C) conditions. The differentially expressed genes (DEGs) were identified to provide key molecular targets for elucidating the mechanisms by which temperature affects molting and development in E. sinensis.
Project description:Reference genome was assembed for mixture sample of Eriocheir sinensis eyestalk under low tempeture (7°C,12°C,17°C) ,normal temperature (22°C) and high temperature (27°C, 32°C) conditions.The reference genome was used for the further transcritome analysis of genes expression profiels in eystalk of Eriocheir sinensis under different tempetures and provide key molecular targets for elucidating the mechanisms by which temperature affects molting and development in E. sinensis.
Project description:The transcriptome profiling five tissues of juvenile Eriocheir sinensis, including gill, muscle, thoracic ganglion, eyestalk and hepatopancreas, were sequenced to get the basic dataset for constructed a genome-scale metabolic network model for E. sinensis. The model was used to predict the optimal nutrient requirements of E. sinensis in feed and suggestions for feed improvement were put forward based on the simulation results.
Project description:We sequenced the mRNA in the Y-organ of normal and eyestalk-ablated Eriocheir Sinensis and obtained the transcriptome profiling (RNA-seq) of the two samples with Next-generation sequencing (NGS) technique. The unigene expression of the two samples were compared and the differetially expressed unigenes were listed.
Project description:Chinese mitten crab (Eriocheir sinensis) is one of the most popular bred crustaceans in China. E. sinensis stressed by high temperature and resulted in disease and large mortality in summer. Litter is known about the mechanism of temperature tolerant E. sinensis. In this study, transcriptome and proteome for high temperature tolerant (HTT) and high temperature sensitive (HTS) female E. sinensis were identified
