Project description:Microarray analysis was performed on the aerial parts of 5 plants collected 24 hours after water treatment or inoculation by the Ralstonia solanacearum hrpB strain (avirulent strain) and 5 days (25% of wilted leaves) after challenge inoculation with the GMI1000 virulent R. solanacearum strain. We compared trancriptome expression level of protected plants (hrpB prinoculation) versus non protected plants (water treatment), 24H after treatment and 5 days (25% of wilted leaves) after challenge inoculation with the GMI1000 virulent R. solanacearum strain
Project description:The aim of the project is to decipher the role of DNA methylation in the plant pathogenic bacteria Ralstonia solanacearum during host adaptation. As a first step, we present here the DNA methylation profile of the GMI1000 reference strain.
Project description:affy_ralstonia_medicago - Ralstonia solanacearum is the causal agent of the devastating bacterial wilt disease. Its infection process was studied with an in vitro inoculation procedure on intact roots of Medicago truncatula. The pathosystem involved susceptible A17 and resistant F83005.5 M truncatula lines infected with the pathogenic strain GMI1000. The mutant A17 line, Sickle, which showed a resistant phenotype was also part of the experiment. To identify host signaling pathway triggered by R. solanacearum infection with a focus on the involvment of ethylene, we used the Medicago Affymetrix array to monitore the expression profiles and the molecular process associated with initial symptoms development (12hpi) and colonization (72hpi). In order to maximize chances to observe differential gene expression, RNA samples were extracted from the root infection zone (root tips) -Three Medicago truncatula lines, A17, F83005.5 and sickle were inoculated with GMI1000 Ralstonai solanacearum strain (107 cfu/ml). RNA were extracted from root extremities (1 cm above the root tip) at time 0, 12h and 72h post inoculation. Three biological repeats were conducted
Project description:Genome sequencing and analysis of Ralstonia solanacearum phylotype I strains FJAT-91, FJAT-452 and FJAT-462 isolated from tomato, eggplant, and chilli pepper in China
Project description:Ralstonia solanacearum can cause devastating bacterial wilt (BW) disease on more than 400 host plants. The pathogenicity debilitation can be found in R. solanacearum under environment stress and subculture. Avirulent R. solanacearum has exhibited good potential for the biocontrol of BW disease. In this study, we will reveal molecular mechanisms underlying pathogenicity debilitation of R. solanacearum by combining transcriptome and proteomic analysis; and provide a new scientific basis for biocontrol of bacteri
Project description:Investigation of whole genome gene expression level changes in the bacterial wilt pathogen Ralstonia solanacearum, strain GMI1000 at 20°C and 28°C in culture and in planta. The tropical strain GMI1000 cannot wilt tomato plants at 20°C although it can cause full-blown disease at 28°C.
