Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Paternal high-fat diet altered sperm 5'tsRNA-Gly-GCC is associated with enhanced gluconeogenesis in the offspring

ABSTRACT: Paternal high-fat diet altered sperm 5'tsRNA-Gly-GCC is associated with enhanced gluconeogenesis in the offspring

PROVIDER: PRJNA807657 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR18052100.fastq.gz	Fastqsanger.gz
	SRR18052091.fastq.gz	Fastqsanger.gz
	SRR18052092.fastq.gz	Fastqsanger.gz
	SRR18052093.fastq.gz	Fastqsanger.gz
	SRR18052094.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 10

Similar Datasets

Paternal high-fat diet altered sperm 5'tsRNA-Gly-GCC is associated with enhanced gluconeogenesis in the offspring

Project description:In this study, we aimed to find out how the sperm tsRNA involved in paternal high-fat diet induced abnormal gluconeogenesis of F1 offspring, and explore the underlying molecular mechanism of its regulation.

2022-02-18 | GSE196881 | GEO

tsRNAs sequencing of FFPE tissues reveals i-tRF-Gly-GCC as a biomarker for colorectal cancer

Project description:Colorectal cancer (CRC) remains one of the most prevalent and deadly malignancies worldwide, requiring reliable biomarkers for detection. This study profiled tRNA-derived small RNAs in CRC tissues, identifying 1051 differentially expressed tsRNAs. Notably, i-tRF-Gly-GCC was significantly elevated in CRC tissues and patient sera compared to controls, with ROC analysis yielding an AUC > 0.7, underscoring its diagnostic potential. Weighted Gene Co-expression Network Analysis (WGCNA) revealed that i-tRF-Gly-GCC's target genes, including RAC2, which was downregulated in the COAD and READ datasets, are closely related to CRC. Gene Ontology and KEGG pathway analyses further linked i-tRF-Gly-GCC to cancer-related processes. Functional experiments in HCT-116 cells demonstrated that i-tRF-Gly-GCC enhances cell proliferation and migration. These results highlight i-tRF-Gly-GCC as a novel non-invasive biomarker for CRC detection and a potential therapeutic target, advancing our understanding of tsRNA-mediated regulation in CRC and paving the way for clinical applications.

2026-04-24 | GSE272805 | GEO

Paternal High-Fat Diet Altered Sperm 5'tsRNA-Gly-GCC Is Associated With Enhanced Gluconeogenesis in the Offspring.

Project description: Not available

| S-EPMC9035875 | biostudies-literature

Regulation of Immature Porcine Sertoli cell proliferation by tRNAGly-GCC and tRNAGlu-CTC-derived small RNAs

Project description:Male fertility is contingent upon spermatogenesis. Sertoli cells, as somatic cells within the seminiferous tubules of the testis, create a specific immune environment conducive to the development of spermatocytes and spermatids, thereby ensuring the smooth progression of spermatogenesis. The proliferation and maturation of Sertoli cells are regulated by non-coding small RNAs. In this study, we explored the expression characteristics and functions of tsRNA in porcine Sertoli cells using small RNA sequencing, immunofluorescence staining, LC-MS/MS, and other techniques. The results of small RNA sequencing indicated that 97.5% of tsRNA in mature porcine Sertoli cells originated from the 5' end of tRNA, with the cleavage site primarily located near the antisense codon, and the length distributed between 31-35 nt. The abundance of tsRNA in porcine Sertoli cells accounted for 4.37% of the total small RNA, which was higher than that observed in spermatogonia and pachytene spermatocytes. Fourteen tsRNA families were found to be highly enriched in the mature Sertoli cells of porcine testis. Three tsRNAs, specifically Gly GCC 2-1, Glu CTC 1-1, and Glu CTC 1-6, were highly enriched in both mature and immature Sertoli cells. Furthermore, YTHDF1 was confirmed as the target gene of Gly GCC 2-1, while CDK1 was identified as the target gene of Glu CTC 1-1. Both Gly GCC 2-1 and Glu CTC 1-1 were shown to reduce the viability of Sertoli cells and inhibit their proliferation. The findings of this study provide a foundational understanding for the development of porcine Sertoli cells and offer a theoretical reference for the regulation of spermatogenesis.

2026-08-23 | GSE275578 | GEO

Serum tsRNA-Gly-5-0006 in breast cancer: Differential expression and effects on cancer cell biology

Project description:Breast cancer (BC) is a leading cause of female mortality worldwide, largely due to metastatic progression and the absence of reliable non-invasive biomarkers for early detection. This study investigates the potential of tRNA-derived small RNA fragments (tRFs) as novel diagnostic and functional molecules in BC. Through high-throughput sequencing and subsequent validation in clinical cohorts, we identified tsRNA-Gly-5-0006 as significantly downregulated in both BC tissues and patient serum compared to adjacent normal tissues, mastitis samples, and healthy controls.

2026-02-14 | GSE319387 | GEO

Clinical utility and biological function of Transfer RNA-derived small RNAs in Patients with Acute Coronary Syndrome [RNA-seq]

Project description:Acute coronary syndrome (ACS) is one of the leading causes of death worldwide. Aberrant expression of Transfer RNA-derived small RNAs (tsRNA) contributed to various human disease. However, the clincal utility and biological function of tsRNA in ACS are largely unknown. High throughput sequencing revealed a landscape of novel sncRNAs in the human PBMCs. tsRNAs represented a characteristic expression pattern in responding to acute coronary syndrome; tRF-Gly-GCC-06 and tRF-Lys-CTT-02 were significantly up-regulated in patients with unstable angina(UA) and acute myocardial infarction(AMI). Both of the two tRFs demonstrated reasonable diagnostic accuracy for ACS detection. tRF-Gly-GCC-06 and tRF-Lys-CTT-02 upregulation were independent predictors of an increased risk of ACS. Both of the two tRFs expression levels show a significantly positive correlation with Gensini score.Our study identified novel tsRNA alterations associated with ACS and provided potential clinical biomarkers and therapeutic targets.

2025-04-30 | GSE279975 | GEO

Clinical utility and biological function of Transfer RNA-derived small RNAs in Patients with Acute Coronary Syndrome [ncRNA-seq]

2025-04-30 | GSE279974 | GEO

tsRNAs sequencing of FFPE tissues reveals i-tRF-Gly-GCC as a biomarker for colorectal cancer

Project description:tsRNAs sequencing of FFPE tissues reveals i-tRF-Gly-GCC as a biomarker for colorectal cancer

| PRJNA1138766 | ENA

Serum tsRNA-Gly-5-0006 in breast cancer: Differential expression and effects on cancer cell biology

Project description:Serum tsRNA-Gly-5-0006 in breast cancer: Differential expression and effects on cancer cell biology

| PRJNA1423213 | ENA

RNA-seq of mouse primary cortical neurons transfected with a synthetic mimic of 5’tiRNA (Gly-GCC) or a scrambled control

Project description:tRNA-derived stress-induced RNAs (tiRNAs) are a new class of small non-coding RNA that have emerged as important regulators of cellular stress responses. tiRNAs are derived from specific tRNA cleavage by the stress-induced ribonuclease angiogenin (ANG). Loss-of-function mutations in the ANG gene are linked to amyotrophic lateral sclerosis (ALS), and elevated levels of specific tiRNAs were recently identified in ALS patient serum samples. However, the biological role of tiRNA production in neuronal stress responses and neurodegeneration remains largely unknown. This study aimed to evaluate the genome-wide regulation of neuronal stress responses by an ALS-associated tiRNA; 5’tiRNAGly-GCC. To this end, we performed whole-transcript RNA-seq on mouse primary cortical neurons transfected with either a synthetic mimic of endogenous 5’tiRNAGly-GCC or a scrambled tiRNA control. This dataset contains the RNA-seq data used to identify the effects of 5’tiRNAGly-GCC on global gene expression in primary neurons.

2025-01-05 | E-MTAB-13537 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data