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RNA sequencing to identify UNC-49-regulated genes in C. elegans defense against pathogen P. aeruginosa infection

ABSTRACT: RNA sequencing to identify UNC-49-regulated genes in C. elegans defense against pathogen P. aeruginosa infection

PROVIDER: PRJNA807900 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18056622_1.fastq.gz	Fastqsanger.gz
	SRR18056622_2.fastq.gz	Fastqsanger.gz
	SRR18056623_1.fastq.gz	Fastqsanger.gz
	SRR18056623_2.fastq.gz	Fastqsanger.gz
	SRR18056624_1.fastq.gz	Fastqsanger.gz

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RNA sequencing to identify UNC-49-regulated genes in C. elegans defense against pathogen infection

Project description:To gain molecular insights on how UNC-49 regulates C. elegans innate immunity, we used RNA sequencing to profile gene expression in unc-49(e407) animals relative to wild-type animals with or without P. aeruginosa(PA14) infection. We found that UNC-49 suppresses the expression of insulin pathway genes, and lack of UNC-49-mediated suppression in unc-49(e407) animals contributes to their improved survival against P. aeruginosa infection.

2023-10-23 | GSE146406 | GEO

RNA sequencing to identify UNC-49-regulated genes in C. elegans defense against pathogen P. aeruginosa infection

Project description:Purpose: To gain molecular insights on how UNC-49 regulates C. elegans innate immunity, we used RNA sequencing to profile gene expression in unc-49(e407) animals relative to wild-type animals with or without P. aeruginosa (PA14) infection. Methods: Synchronized L1 worms were placed onto NGM plates seeded with E. coli OP50 and grown at 20 °C until the L4 stage. Worms were washed off the plates, collected with M9 solution and subsequently transferred to modified NGM plates containing E. coli OP50 or P. aeruginosa PA14 for 24 h at 25 °C. Then, the animals were washed off the plates with M9 solution and frozen in TRIzol (Transgen, ER501-01, China) with liquid nitrogen. Total RNA was isolated using a TransZol Up Plus RNA kit (Transgen, ER501-01, China). Results: RNA seq analyses shows enriched and signficant changed immune genes and pathways that are dependent of GABAergic neuromuscular junctions. Conclusion: Our studies uncover that lack of UNC-49-mediated suppression in unc-49(e407) animals contributes to their improved survival against P. aeruginosa infection.

2025-02-17 | GSE196933 | GEO

RNA sequencing to identify UNC-49-regulated genes in C. elegans defense against pathogen infection

Project description:RNA sequencing to identify UNC-49-regulated genes in C. elegans defense against pathogen infection

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RNA sequencing to identify UNC-25-regulated genes in C. elegans defense against pathogen P. aeruginosa infection

Project description:Purpose: To gain molecular insights on how UNC-25 regulates C. elegans innate immunity, we used RNA sequencing to profile gene expression in unc-25(e156) animals relative to wild-type animals with or without P. aeruginosa (PA14) infection. Methods: Synchronized L1 worms were placed onto NGM plates seeded with E. coli OP50 and grown at 20 °C until the L4 stage. Worms were washed off the plates, collected with M9 solution and subsequently transferred to modified NGM plates containing E. coli OP50 or P. aeruginosa PA14 for 24 h at 25 °C. Then, the animals were washed off the plates with M9 solution and frozen in TRIzol (Transgen, ER501-01, China) with liquid nitrogen. Total RNA was isolated using a TransZol Up Plus RNA kit (Transgen, ER501-01, China). Results: RNA seq analyses shows enriched and signficant changed immune genes, neuropeptides, transcription factors and pathways that are dependent of GABA signaling. Conclusion: Our studies uncover that GABA signaling enhance the resistence to pathogen induced killing.

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RNA-Seq to identify NMUR-1-regulated genes in C. elegans defense against pathogen infection

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