Project description:We employed a proteogenomics workflow to identify microproteins encoded by small Open Reading Frames (ORFs) in the genome of Mycobacterium smegmatis strain mc²155.
Project description:This study performed transcriptome sequencing of two newly identified Mycobacterium species, Mycobacterium camsae and Mycobacterium pumcae, under standard culture conditions to establish baseline gene expression profiles. RNA was extracted from cells grown to mid-log phase in standard medium and sequenced using Illumina paired-end technology. The data provide a foundational resource for future functional studies of these novel strains.
Project description:Related surrogate species are often used to study the molecular basis of pathogenicity of a pathogen on the basis of a shared set of biological features generally attributable to a shared core genome consisting of orthologous genes. An important and understudied aspect, however, is the extent to which regulatory features affecting the expression of such shared genes are present in both species. Here we report on an analysis of whole transcriptome maps for an important member of the TB complex Mycobacterium bovis and a closely related model organism for studying mycobacterial pathogenicity Mycobacterium marinum.
