Project description:To validate the use of chicken array for turkey, the ability of species-specific hybridization (SSH, chicken samples-chicken arrays) and cross-species hybridization (CSH, turkey samples-chicken arrays) were compared in the same biological conditions. Reproductively active laying chickens and reproductively inactive non-laying pullets were used to generate the results for SSH. Similarly, reproductively active laying turkeys and reproductively inactive non-laying photorefractory turkeys were used to generate the results for CSH.
Project description:The objective of this study was to determine differential gene expression of turkey breast muscle regarding development of PSE meat defect. Genetically unimproved, random-bred (RBC2) turkeys representing turkeys from 1966, which are smaller and grow slower than modern turkeys, were raised at the Michigan State University (MSU) Poultry farm and harvested at 22 week of age. Breast meat was collected and snap frozen in liquid nitrogen. Percent marinade uptake at 24h post-slaughter of each sample was determined. The highest (n=6) and the lowest (n=6) marinade uptake were classified as normal and PSE, respectively. Differentially expressed genes between normal and PSE was identified using TSMLO microarray and confirmed by qRT-PCR. Forty-one oligos were differentially expressed (false discovery rate, FDR<0.1). Candidate genes and pathways associated with development of PSE in turkey were suggested for further experiment to gain greater comprehension about this meat quality defect.
Project description:Our results indicated that conditional knockout of Cdc42 in the epithelial cells of the oviduct isthmus led to defective embryo transport, resulting in blastocytes retention in oviduct, and contributing to the pregnancy failure in PgrCreCdc42f/f mice. To further determine the role of CDC42 in oviduct, RNA-seq analysis was applied in the Cdc42f/f and PgrCreCdc42f/f mice oviduct on day 2 of pregnancy.
Project description:While previous studies have reported in vitro formation of oviduct epithelial spheroid models in porcine species (sus scrofa), these approaches often rely on manual selection of the formed aggregates. Here, we establish a scalable and reproducible system for generating oviduct spheroids. We describe steps for the isolation of primary secretory and ciliated oviduct epithelial cells and their assembly into spheroids using the AggreWell platform. This approach enables the mass production of uniformly sized spheroids ready for use in experiments such as sperm-oviduct co-incubation experiments.
Project description:We developed a genetically engineered conditional compound heterozygous Dicer1 mouse strain that fully recapitulates the bi-allelic mutations of DICER1 in DICER1 syndrome-associated cancers. Embryonic activation of bi-allelic Dicer1 mutations, driven by the anti-Müllerian hormone receptor 2 (Amhr2)-driven Cre strain (Amhr2+/cre), drove cancer development from oviduct. Small RNA sequencing was performed to compare the microRNA expression profiles between tumor and normal oviduct.
