Project description:To undestand the mechanism involved in abitoic stress tolerance of P. Putida (NBAII-RPF 9) the bacterium was grown in liquid LB media overnight and further subjected to saline shock of (1M NaCl) for one hour seperately. The cultures were pelleted with centrifuged for total RNA. The RNA was hybridised in 8X15K Agilent array and image analysis carried out with Agilent Microarray scanner.
Project description:To undestand the mechanism involved in abitoic stress tolerance of P. Putida (NBAII-RPF 9) the bacterium was grown in liquid LB media overnight and further subjected to heat shock at 45 degrees in incubated shaker for one hour seperately. The cultures were pelleted with centrifuged for total RNA. The RNA was hybridised in 8X15K Agilent array and image analysis carried out with Agilent Microarray scanner
Project description:This study investigated the proteomic response of Staphylococcus aureus ATCC 25923 to dopamine. Strains were cultured in LB medium with or without dopamine supplementation. After 6 hours of incubation, bacterial cells were collected for quantitative proteomic analysis to screen differentially expressed proteins and explore the regulatory mechanism of dopamine on this bacterium.
Project description:A phoP mutant of the entomopathogenic bacterium P. luminescens is attenuated in virulence in insects and susceptible to antimicrobial peptides such as Polymyxin B. The first goal of this study is to compare transcriptomes of the phoP mutant and wild type strain to identify the PhoP regulon. (i) We first compared both strains grown in LB medium. (ii) As we know that low Mg conditions induce expression of PhoP-dependent genes, we also compared transcriptomes of the phoP and wild-type strain after growth in M9 low Mg and in M9 High Mg. To decipher the polymyxin B regulon, comparisons of transcriptomes of the wild type strain grown in LB medium with or without addition of polymyxin B were also performed
Project description:It has been performed a genome-wide analysis of gene expression of the root-colonizing bacterium Pseudomonas putida KT2440 in the rhizosphere of corn (Zea mays var. Girona. To identify reliable rhizosphere differentially expressed genes, rhizosphere populations of P. putida bacteria cells were compared with three alternative controls: i) planktonic cells growing exponentially in rich medium (LB), ii) planktonic cells in stationary phase in LB, and iii) sessile populations established in sand microcosms, under the same conditions used to grow inoculated corn plants.
