Project description:CX3CR1pos monocytes are mobilized upon infection and undergo monocyte-to-macrophage transition in inflamed tissues. Using scRNA-seq of CD11c+ cells from bronchoalveolar lavage fluid (BALF) infected with IAV (PR/8, H1N1 ,we demonstrate that, during severe viral pneumonia, bone marrow-derived macrophages (BMDM) pass co-ordinated trajectories of pro-inflammatory-to-tissue-healing phenotypes, before differentiating into tissue-resident alveolar macrophages, that retain a long-term tissue-protective phenotype.
Project description:To delineate the single-cell transcriptional landscape of the lungs in severe patients infected with the Omicron variant of SARS-CoV-2, we performed single-cell RNA sequencing on bronchoalveolar lavage fluid samples collected within 24 hours of ICU admission.
Project description:This study aimed to delineate molecular phenotypes of the lung microenvironment across idiopathic interestitial pneumonias, namely interstitial pneumonia with autoimmune features (IPAF)and idiopathic pulmonary fibrosis (IPF) through proteomic analysis of bronchoalveolar lavage fluid (BALF).
Project description:Sepsis-induced acute lung injury (ALI) is prevalent in septic patients and has a high mortality rate. Considering ALI’s close link to sepsis, we used a Pseudomonas aeruginosa (PA) pneumonia-induced sepsis mouse model to investigate alveolar microenvironment alterations and lung injury post-sepsis. Peptidyl arginine deiminase (PADI) 2 and PADI4 are highly expressed in immune cells, and play substantial roles in the immune response to sepsis, but their specific functions remain unclear.We employed single-cell RNA sequencing (scRNA-seq) technology to map immune cell populations in bronchoalveolar lavage fluid (BALF) cells from Wild type (WT) and Padi2 and Padi4 double knock-out (DKO)mice in PA pneumonia-induced sepsis and sham conditions.