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Transcriptomes of Enterococcus faecalis OG1RF and OG1RF Δbph in planktonic culture

ABSTRACT: Transcriptomes of Enterococcus faecalis OG1RF and OG1RF Δbph in planktonic culture

PROVIDER: PRJNA813528 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR18255491_1.fastq.gz	Fastqsanger.gz
	SRR18255491_2.fastq.gz	Fastqsanger.gz
	SRR18255492_1.fastq.gz	Fastqsanger.gz
	SRR18255492_2.fastq.gz	Fastqsanger.gz
	SRR18255493_1.fastq.gz	Fastqsanger.gz

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Transcriptomes of Enterococcus faecalis OG1RF and OG1RF Δbph in planktonic culture

Project description:Liquid cultures of Enterococcus faecalis OG1RF and OG1RF Δbph were grown in tryptic soy broth without added dextrose (TSB-D) for 2 and 4 hr. At each time point, the transcriptomes were compared to identify differentially expressed genes in the Δbph mutant.

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Genome-wide Analysis of Enterococcus faecalis OG1RF Gene Expression During Infection

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Project description:Changes in Enterococcus faecalis OG1RF gene expression during infection in a rabbit model of subdermal abscess formation were studied using microarray analysis. Samples assayed in arrays consisted of the input inoculum and total RNA collected from the subdermal chambers at 2 and 8 hours post-inoculation. Alexa647-labeled samples were co-hybridized with 0.5 micrograms of sheared OG1RF genomic DNA labeled with Cy3 as a standard reference between chips. Four biological replicates were performed for each of the 3 time points.

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