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RNAseq analysis of the mouse aorta in response to Estradiol, Estetrol and Tamoxifen

ABSTRACT: RNAseq analysis of the mouse aorta in response to Estradiol, Estetrol and Tamoxifen

PROVIDER: PRJNA813842 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18270906_1.fastq.gz	Fastqsanger.gz
	SRR18270906_2.fastq.gz	Fastqsanger.gz
	SRR18270907_1.fastq.gz	Fastqsanger.gz
	SRR18270907_2.fastq.gz	Fastqsanger.gz
	SRR18270908_1.fastq.gz	Fastqsanger.gz

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RNAseq analysis of the mouse aorta in response to Estradiol, Estetrol and Tamoxifen

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Project description:Assessment of different subcellular localizations (membrane, cytosol, nucleus) of estrogen receptors by multi-epitope protein expression in 200 NSCLC specimens and matched non-cancerous tissues and ESR-1 probe mapping meta-analysis of Affymetrix 2.0 plus data in 1398 NSCLC tumors and normal lung tissues and 39 NSCLC cell lines led us to the conclusion that ERα extranuclear variants are the main ERs in NSCLC. In order to further analyze these effects we treated A549 and H520 cell lines with 17β-estradiol, 17β-estradiol-BSA (plasma membrane impermeable conjugate) and tamoxifen for 3h, in order to investigate early transcriptional effects and responsiveness to estrogen agonists and antagonists. The aim of this study was to demonstrate the functionality of NSCLC ERs and decipher which genes, canonical pathways and networks are affected by each treatment.

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