Project description:In this experiment, we studied how human beta defensin-2 (hBD-2) affects gene expression of T cells. Murine splenic C57BL/6 CD4/CD8 T cells were stimulated with anti-CD3/anti-CD28 beads and hBD-2 or vehicle (PBS) control for 48h before RNA isolation
Project description:Rhesus theta defensin (RTD)-1, a cyclic antimicrobial peptide, regulates gene expression and immune signaling pathways in cell culture and animal models of immune mediated diseases. To gain insights into RTD-1 regulation of naïve cells, we performed RNA sequencing (RNA-seq) to determine the effect of RTD-1 on global gene expression in human monocytes. Analysis of differentially expressed genes revealed RTD-1 mediated stimulation of interferon and antiviral gene expression pathways in human monocytes.