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Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet

ABSTRACT: Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet

PROVIDER: PRJNA815853 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18318559_1.fastq.gz	Fastqsanger.gz
	SRR18318559_2.fastq.gz	Fastqsanger.gz
	SRR18318560_1.fastq.gz	Fastqsanger.gz
	SRR18318560_2.fastq.gz	Fastqsanger.gz
	SRR18318561_1.fastq.gz	Fastqsanger.gz

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Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet

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Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet.

Project description:The response of vital organs to different types of nutrition or diet is a fundamental question in physiology. We examined the cardiac response to 4 weeks of high-fat diet in mice, measuring cardiac metabolites and mRNA. Metabolomics showed dramatic differences after a high-fat diet, including increases in several acyl-carnitine species. The RNA-seq data showed changes consistent with adaptations to use more fatty acid as substrate and an increase in the antioxidant protein catalase. Changes in mRNA were correlated with changes in protein level for several highly responsive genes. We also found significant sex differences in both metabolomics and RNA-seq datasets, both at baseline and after high fat diet. This work reveals the response of a vital organ to dietary intervention at both metabolomic and transcriptomic levels, which is a fundamental question in physiology. This work also reveals significant sex differences in cardiac metabolites and gene expression.

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Project description:In this study, we investigated the dynamic changes of intramuscular fat (IMF) deposition in the breast muscle of Jingyuan chickens at different stages (42-, 126- and 180-day-old) using metabolomics. pH45min, a*, and L* were significantly increased in the breast muscle of 126-day-old chickens, and IMF content and b* were significantly increased in the breast muscle of 180-day-old chickens. A total of 4,643 differentially expressed metabolites (DEMs) were identified, of which 10 decreased and 29 increased with age. Key pathways associated with meat quality included oxidative phosphorylation, β-alanine metabolism, and glycerophospholipid metabolism. Combined transcriptomic and phenotypic correlation analyses revealed significant positive correlations of IMF, pH45min, a*, and L* with LysoPC 20:4, CD3E, TARP, IL7R, ENSGALG00010025331, and RASSF5. In the 42- and 180-day-old chickens, IMF, pH45min, a*, and L* were significantly and positively correlated with L-Anserine, Dihydroxyacetone phosphate, ENSGALG00010006904, and HSPB7. IMF, pH45min, a*, and L* were significantly and positively correlated with beta-Nicotinamide adenine dinucleotide in the 126- and 180-day-old. This study deepens our understanding of the differences in IMF deposition at different stages of Jingyuan chickens and its relationship with meat quality.

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