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Epigenome analysis of glycine chloramine treated Jurkat T cells

ABSTRACT: Epigenome analysis of glycine chloramine treated Jurkat T cells

PROVIDER: PRJNA815983 | ENA |

REPOSITORIES: ENA

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Epigenome analysis of glycine chloramine treated Jurkat T cells

Project description:Genome wide DNA methylation profiling of Jurkat T lymphoma cell lines, using the human DNA methylationEPIC 850K array. All cells were blocked using a thymidine block, and DNA methylation was analysed 4 hrs post release from the block (S-Phase) or 72 hours later. Samples are either untreated control (PBS) or treated with Glycine chloramine. Cells were exposed to Glycine chloramine in two independent time points: pre replication (same time as release from block, 3 replicates) or during S-phase (four replicates, 2 hrs post block release). These two exposures were assessed in two independent experiments and therefore have their own controls. Each replicate used cells from a different passage number, and was performed independently.

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Epigenome and gene expression analysis of glycine chloramine treated Jurkat T cells

Project description:Epigenome and gene expression analysis of glycine chloramine treated Jurkat T cells

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Project description:The goal of the current study was to determine whether chloramine exposure results in long-term alterations in transcriptional output. Proliferating Jurkat T-lymphoma cells were exposed to sublethal doses of glycine chloramine and RNA expression was profiled at 4, 24, 48 and 72 hours.

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Glycine Chloramine directed gene expression changes in Jurkat T cell lines

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Next Generation Sequencing Facilitates Quantitative Analysis of primary myoblast cell treated with different concentration glycine Transcriptomes

Project description:Purpose: The goals of this study are to analyze primary myoblast cell from mdx mice skeletal muscle transcriptome profiling (RNA-seq) treated with different concentration glycine. Methods: primary myoblast cell isolated from 6-8-week-old mdx mice limb skeletal muscle and the primary myoblast treated with 0.2mM or 0.8mM glycine 24-hour. Primary myoblast mRNA profiles of 0.2mM glycine treated(3 replicates) and 0.8mM glycine treated(6 replicates) were generated by deep sequencing, using Illumina HiSeq 2500. The sequence reads that passed quality filters were analyzed at the gene level using HISAT2 and StringTie followed by DESeq2.

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Epigenome and gene expression analysis of glycine chloramine treated Jurkat T cells

Project description:This SuperSeries is composed of the SubSeries listed below.

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Differential expression of miRNA in control and CD3/CD28mAb-treated Jurkat cells

Project description:The cultured cell line Jurkat is frequently employed in studies of T cell function. Here we identified the microRNAs expressed in Jurkat cells in the presence and absence of CD3/CD28mAb treatment. Analyzed the expression of microRNAs extracted from untreated Jurkat cells as a control and Jurkat cells treated with CD3/CD28mAb.

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Project description:To finely dissect the dynamic changes in the epigenome and chromatin state during T cell activation, we utilized the Jurkat cell line as a model and performed ChIP-seq on both resting and anti-CD3/CD28-stimulated Jurkat cells, targeting various histone modifications (H3K27ac, H3K4me1, H3K4me3, H3K27me3), RNA PolII, and CTCF.

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