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Analysis of wild type and Sema6d-/- lung ILC2 transcriptomes in IL-33 induced acute lung inflammation

ABSTRACT: Analysis of wild type and Sema6d-/- lung ILC2 transcriptomes in IL-33 induced acute lung inflammation

PROVIDER: PRJNA816419 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18330972.fastq.gz	Fastqsanger.gz
	SRR18330973.fastq.gz	Fastqsanger.gz
	SRR18330974.fastq.gz	Fastqsanger.gz
	SRR18330975.fastq.gz	Fastqsanger.gz
	SRR18330976.fastq.gz	Fastqsanger.gz

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Analysis of wild type and Sema6d-/- lung ILC2 transcriptomes in IL-33 induced acute lung inflammation

Project description:Semaphorin 6D (Sema6D) have various effects in immune responses. However, the involvement of Sema6D in ILC2 funcions remains unclear. We identified the genes regulated by Sema6D in lung ILC2s during IL-33 induced acute lung inflammation.

2022-08-28 | GSE198659 | GEO

Human ILC2 cells stimulated with IL-25, IL-33, or TSLP

Project description:ILC2 cells are a newly described cell type whose biology and contribution to disease are poorly understood. ILC2 cells are activated by allergens, viral infection, and/or epithelial damage via IL-33 and IL-25. ILC2 cells require IL-2, IL-7, IL-25 and IL-33 for their survival and expansion. In mice, ILC2s produce multiple mediators primarily associated with type 2 inflammation (IL-13, IL-5, IL-4, IL-6, IL-9, IL-10, GM-CSF, amphiregulin). ILC2 cells may contribute to the pathology of asthma through multiple mediators that include IL-13-independent pathways. Our goal is to compare transcriptional profiles of IL-33- or IL-25-activated ILC2 cells from blood to characterize these cells and to identify marker(s) that can be utilized to detect them in human tissue. ILC2 cells (Lineage negative, CRTH2+, CD161+, CD127+) were purified from human blood of 5 different donors by flow cytometry. The ILC2 yield ranged from 20,000 to 165,000 cells per donor (0.001-0.008% WBC). Purified ILC2s were expanded in vitro in the presence of IL-2, IL-7, IL-33 and IL-25 (each at 50 ng/ml) for 7-10 days. Expanded cells maintained the ILC2 phenotype (Lineage negative, CRTH2+, CD161+, CD127+). The cells were rested for 2 days in the presence of 1 ng/ml IL-2 and IL-7 and then treated in the presence of 1 ng/ml IL-2 and IL-7 with either media control, IL-25 (50 ng/ml), IL-33 (50 ng/ml), and/or TSLP (50 ng/ml) in combination, for 6 or 24 hours. Whole RNA was isolated via the RNeasy kit (Qiagen). Stratagene Universal Human Reference RNA was used as the reference.

2013-12-31 | E-GEOD-45458 | biostudies-arrayexpress

Human ILC2 cells stimulated with IL-25, IL-33, or TSLP

2013-12-31 | GSE45458 | GEO

Homeostatic leukotrienes, IL-25, and IL-33 signals on intestinal ILC2 status

Project description:We report the high-throughput profiling of murine intestinal type 2 innate lymphoid cells (ILC2) transcriptome. By obtaining over 8.7 million bases of sequence, we generated genome-wide expression maps of mouse ILC2 without the presense of known activation signals(IL-25, IL-33 receptor) and new non-redundant signal (leukotriene). We find that homeostatic signaling minimally contributes to intestinal ILC2 activation statu. 500 ILC2s were sorted as CD45+Lineage-IL-17RB+

2020-02-16 | GSE144956 | GEO

Role of IL-33/ILC2 in bladder infection

Project description:The impact of IL-33 on urinary tract infections (UTI) was assessed by comparing Il33KO mice with wild-type (WT) mice within the bladder;The effect of ILC2 on UTI was assessed by comparing Il5cre+/DTA+ mice with WT mice within the bladder.

2024-10-20 | GSE259362 | GEO

Transcriptome analysis of acute IL-33 or chronic HDM activated murine lung ILC2s

Project description:We have identified CD25high and CD25low ILC2 subsets in various mouse models of airway inflammation. Transcriptome analysis of acute IL-33 (CD25High) or chronic HDM (CD25Low) activated murine lung ILC2s suggests functional differences between these two ILC2 populations.

2020-05-13 | GSE99780 | GEO

Role of IL-33/ILC2 in bladder infection

Project description:Role of IL-33/ILC2 in bladder infection

| PRJNA1081360 | ENA

RNA-sequencing of microglia, T cells and ILC2 from the brains of IL-33 treated aged (20-22mos) mice

Project description:ILC2 are potent producers of IL-5 and IL-13 and Th2 cytokines have been found to promote neurogenesis and cognitive functions. We report microglia, brain T cells and ILC2 have a range of differentially expressed genes in IL-33 treated aged mice. Brain ILC2 upregulated a range of neuroprotective genes.

2020-01-21 | GSE129996 | GEO

Gene expression data of naïve wildtype and IL-33 knockout ILC2s isolated from adult male mouse lungs

Project description:Group 2 innate lymphoid cells (ILC2s) in mouse lungs are activated by the epithelium-derived alarmin IL-33. Activated ILC2s proliferate and produce IL-5 and IL-13 that drive allergic responses. In neonatal lungs, IL-33 is spontaneously released resulting in activation of lung ILC2s. Here we report that neonatal lung ILC2 activation has significant effects on ILC2 functions in adulthood. Most neonatal lung ILC2s incorporated bromodeoxyuridine (BrdU) and persisted into adulthood. BrdU+ ILC2s in adult lungs responded more intensely to IL-33 treatment than BrdU- ILC2s. In IL-33 deficient (KO) mice, lung ILC2s develop normally but they are not activated in the neonatal period. Lung ILC2s in KO mice responded less intensely to IL-33 in adulthood compared to wild type (WT) ILC2s. While there was no difference in the number of lung ILC2s, there were fewer IL-13+ ILC2s in IL-33KO than IL-33WT mice. The impaired responsiveness of ILC2s in KO mice was reversed by intranasal injections of IL-33 in the neonatal period. These results suggest that activation of lung ILC2s by endogenous IL-33 in the neonatal period may “train” ILC2s seeding the lung after birth to become long-lasting resident cells that respond more efficiently to challenges later in life.

2020-04-14 | GSE148545 | GEO

scRNA-sequencing of ILC2 sorted from the choroid plexus and meninges of IL-33 treated aged (20-22 mos) mice

Project description:ILC2 are potent producers of IL-5 and IL-13 and Th2 cytokines. We have found that they promote neurogenesis and cognitive functions in aged mice . We report that CP and meningeal ILC2 from IL-33 treated aged mice have differential expression of Th2 cytokines as well as genes involved in metabolism through scRNA-sequencing.

2020-01-21 | GSE139328 | GEO

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