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Identification of candidate regulatory elements controlling transcriptome during the formation of interphalangeal joints

ABSTRACT: Identification of candidate regulatory elements controlling transcriptome during the formation of interphalangeal joints

PROVIDER: PRJNA816976 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18354919.fastq.gz	Fastqsanger.gz
	SRR18354920.fastq.gz	Fastqsanger.gz
	SRR18354921.fastq.gz	Fastqsanger.gz
	SRR18354922.fastq.gz	Fastqsanger.gz
	SRR18354923.fastq.gz	Fastqsanger.gz

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Identification of candidate regulatory elements controlling transcriptome during the formation of interphalangeal joints

Project description:We report the ChIPseq derived Candidate Enhancer (CE) atlas generated for interphalangeal interzone and phalange, tissues collected during synovial joint formation. Three replicates for interzone and three replicated for phalange were immunoprecipitated (IP) with the H3K27ac antibodies. Also, two replicates for interzone and two replicates for phalange were immunoprecipitated with H3K4me1 antibodies. The IP samples for both histone marks were sequenced and used for annotation of CEs. Further, the CEs were functionally annotated. In parallel, the total RNA for three interzone and three phalange replicates was isolated and mRNA sequencing libraries were constructed. Next the identification of differentially expressed genes (DEGs) and integrative analysis of DEGs and CEs was performed.

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