Project description:Transcription profiling by array of the response of Arabidopsis cultivar Columbia etiolated seedlings and undifferentiated tissue culture cells to the spaceflight environment
Project description:During initial colonization of the airways, MAH form microaggregates composed of 3-20 bacteria on human respiratory epithelial cells, which provides an environment for phenotypic changes leading to efficient mucosal invasion. DNA microarray was employed to identify genes associated with the microaggregate phenotype. Bacteria were incubated with Hep-2 epithelial cells for 24 hrs to form microaggregates or incubated in tissue culture media alone as the control (planktonic bacteria). Bacterial RNA was isolated, purified using MicrobeEnrich, and amplified using Abmbion's Bacterial MessageAMP kit. RNA was hybridized to Affymetrix custom made mycobacterium avium 104 microarrays
Project description:Bacteria that live in the acidic environment face number of growth-related challenges from the intracellular pH changes. In order to survive under acidic environment, Lactic acid bacteria must employ multiple genes and proteins to regulate the relative pathways.
Project description:Bacteria that live in the acidic environment face number of growth-related challenges from the intracellular pH changes. In order to survive under acidic environment, Lactic acid bacteria must employ multiple genes and proteins to regulate the relative pathways.
Project description:Individuals with cystic fibrosis are susceptible to co-infection by Aspergillus fumigatus and Pseudomonas aeruginosa, however P. aeruginosa eventually predominates as the primary pathogen. Several factors are likely to facilitate P. aeruginosa colonization in the airways, including alterations to the microbial environment. In this study, significant growth proliferation was observed in P. aeruginosa when the bacteria were exposed to culture filtrates produced by A. fumigatus in a nitrate-rich, nutrient-poor liquid broth, Czapek-Dox. Proteomic analysis of the A. fumigatus culture filtrate identified a significant number of environment-altering proteases and peptidases, which may contribute to the growth promoting effect observed when P. aeruginosa is exposed to this culture filtrate. These findings offer insights into the determinants that contribute to P. aeruginosa proliferation in the presence of A. fumigatus.
Project description:Transcriptional profiling of Caco-2 cells comparing Caco-2 monolayers cultured in a custom built co-culture chamber, either inside a 5% CO₂ incubator (conventional cell culture environment) or an anaerobic workstation (apical anaerobic environment) for 12 hours.
