Project description:Transcriptome analysis of two population of peritoneal mononuclear phagocytes (CD14+ macrophages and CD1c+ dendritic cells) in peritoneal dialysis effluent from stable (infection-free) peritoneal dialysis patients.
Project description:Peritoneal mesothelial cells are harmed by peritoneal dialysis fluids (PDF) used in renal replacement therapy with peritoneal dialysis. The mechanisms of the cellular damage are not yet described in detail. Primary human peritoneal mesothelial cells derived from omentum of five donors were independently exposed to peritoneal dialysis fluids (extended recovery time). The extent of cell damage was assessed using lactate dehydrogenase (LDH) release in the cell culture supernatant and cells were lysed in order to extract mRNA and proteins. Transcriptional changes induced by PDF were analyzed using gene expression microarrays and changes of the proteome were analyzed using 2D-electrophoresis.
Project description:Peritoneal mesothelial cells are harmed by peritoneal dialysis fluids (PDF) used in renal replacement therapy with peritoneal dialysis. The mechanisms of the cellular damage are not yet described in detail. Primary human peritoneal mesothelial cells derived from omentum of five donors were independently exposed to peritoneal dialysis fluids. The extent of cell damage was assessed using lactate dehydrogenase (LDH) release in the cell culture supernatant and cells were lysed in order to extract mRNA and proteins. Transcriptional changes induced by PDF were analyzed using gene expression microarrays and changes of the proteome were analyzed using 2D-electrophoresis.
Project description:Peritoneal mesothelial cells are harmed by peritoneal dialysis fluids (PDF) used in renal replacement therapy with peritoneal dialysis. The mechanisms of the cellular damage are not yet described in detail. Primary human peritoneal mesothelial cells derived from omentum of five donors were independently exposed to peritoneal dialysis fluids. The extent of cell damage was assessed using lactate dehydrogenase (LDH) release in the cell culture supernatant and cells were lysed in order to extract mRNA and proteins. Transcriptional changes induced by PDF were analyzed using gene expression microarrays and changes of the proteome were analyzed using 2D-electrophoresis.
Project description:The complete systemic deregulated biological network in peritoneal dialysis (PD) patients is still only partially defined. High-throughput/omics techniques may offer the possibility to analyze the main biological fingerprints associated with this clinical condition. For the transcriptomic part of the study, we analyzed new data from 10 patients undergoing peritoneal dialysis .
Project description:Cardiovasculopathy is the leading cause of death; patients with chronic kidney disease (CKD) are at particularly high risk. The pathophysiological role of accumulating reactive metabolites in CKD such as glucose degradation products (GDP) is uncertain. Large amounts of GDP are absorbed from conventional dialysis fluids in patients on chronic peritoneal dialysis (PD). Omental and parietal peritoneal tissues were obtained from 107 pediatric patients with CKD5 and 90 children on PD using dialysis fluids containing low and high concentrations of GDP, respectively. Fat surrounded omental arterioles, protected from local PD fluid exposure, were microdissected for transcriptome and proteome analyses.
Project description:Peritoneal dialysis related fibrosis (PD-related fibrosis) is one of the most common complications of PD and one of the main reasons for patients to stop PD, occurring almost exclusively in all long-term PD patients. The mechanism of PD-related fibrosis is currently not fully understood. Previous studies have shown that it is related to frequent peritonitis and the epithelial-mesenchymal transition (more accurately, mesothelial-mesenchymal transition) of peritoneal mesothelial cells caused by high glucose and high osmotic non physiologically compatible PD fluid. Due to the lack of effective prevention and treatment methods for PD-related fibrosis, the study of its mechanism is of great clinical significance. Collecting dialysis fluid from patients after 3 years of PDfor single-cell sequencing can help observe changes in the peritoneal environment and structural composition during dialysis, providing a new perspective for the prevention and treatment of PD-related fibrosis.
Project description:Peritoneal fibrosis is regard as a significant cause of the loss of peritoneal function, markedly limiting the application of peritoneal dialysis(PD). However, the pathogenesis of peritoneal fibrosis remains unclear. Tissue-derived extracellular vesicles(EVs) mediate intercellular communications and play a central role in organ fibrosis. We performed a proteomics profiling of EVs from normal peritoneum and PD-induced fibrotic peritoneum in mice.