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Transcriptional profile of Pseudomonas aeruginosa infected cells

ABSTRACT: Transcriptional profile of Pseudomonas aeruginosa infected cells

PROVIDER: PRJNA819977 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18483120_1.fastq.gz	Fastqsanger.gz
	SRR18483120_2.fastq.gz	Fastqsanger.gz
	SRR18483121_1.fastq.gz	Fastqsanger.gz
	SRR18483121_2.fastq.gz	Fastqsanger.gz
	SRR18483122_1.fastq.gz	Fastqsanger.gz

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Transcriptional profile of Pseudomonas aeruginosa infected cells

Project description:Transcriptional profile of Pseudomonas aeruginosa infected cells

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Transcriptional modulation of Quoring-sensing signaling throught QslA in Pseudomonas aeruginosa PA14 and PAO1.

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Project description:To investigate the gene expression profile of pellicle cells of Pseudomonas aeruginosa, microarray analysis was performed. Transcriptome profiles of pellicle cells and planktonic cells grown in LB medium were determined by Affymetrix GeneChip. Gene expression pattern that is specific to pellicle cells was evaluated by comparing the data set with that of planktonic cells. Pseudomonas aeruginosa wild type (PAO1ut) strain was cultivated aerobically in LB in Erlenmeyer flasks under static or shaking conditions, and total RNAs were extracted at 24 hours (static culture) and early stationary phase (OD600 = 1.4, shaking culture). The experiment was performed in duplicate independent cultures.

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Transcriptional response of Pseudomonas aeruginosa PAO1 to NaClO

Project description:In this experiment the transcriptional response of the opportunistic human pathogen Pseudomonas aeruginosa to sublethal concentrations of NaClO was investigated. To this aim, four independent cultures of P. aeruginosa PAO1 grown in minimal medium BM2 were treated with NaClO (2 ug/ml) for 1 h at 37 C followed by RNA extraction and microarray analysis. Untreated cultures served as controls.

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RNA Sequencing of unc-30(ok613) and wild type (WT) N2 C. elegans in Pseudomonas aeruginosa (PA14) infection

Project description:Purpose: To uncover immune mediated genes and pathways by Pseudomonas aeruginosa infection that are modulated by the homeodomain PITX1/UNC-30, which plays a vital role in the GABAergic signaling in C. elegans Methods: RNA was extracted from synchronized Pseudomonas aeruginosa infected L4 stage unc-30(ok613) and WT using Qiagen extraction kits and following standard methods. The animals were grown on OP50 at 20 C and infected at 25 C. Results: RNA seq analyses shows enriched and signficant Pseudomonas aeruginosa mediated upregulated immune, neuropeptide and metabolism genes and pathways that are dependent of GABAergic signaling Conclusions: Our study uncovered GABAgergic signaling to be modulator of the innate immunity in C elegans during Pseudomonas aeruginosa infection

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